• Geeta Dhania Bahamnia

High frequency callus mediated rapid shoot organogenesis was induced in 2d old coyledonary explants of Brassica juncea cv Pusa bold, inoculated onto the MS media supplemented with very low concentration of thidiazuron (N-phenyl-N’-1, 2, 3-thidiazol-5-yl) urea) TDZ (0.1-1 µM). Highest number of Shoots (14-15) regenerated in vitro were obtained with 0.5 µM TDZ. This morphogenetic response of B.juncea is genotype dependent, amongst the other cultivars tested e.g. pusa bold,pusa bahar,9304,luxmi,T-59,B9,RH-30 and PJK,Pusa bold found to be most responsive both in regeneration frequency and in number of shoots/explants. Of the four explants e.g. hypocotyls, shoot tip, cotyledon and cotyledonary node, cotyledon produced maximum shoots/explants. The calli produced by 2 day old cotyledons of B. juncea on TDZ were subcultured twice on the same media, which produced about 8-9 and 5-6 shoots in 1st and 2nd subculture respectively. The regenerated shoots were rooted with 100% frequency on MS media supplemented with Indole-3-butyric acid (IBA; 1 µM) and mature fertile plants were developed in pots which showed a normal phenotype. Our experiments indicate that TDZ is a very potential factor to induce a rapid, high frequency shoot regeneration from young cotyledonary explants via a callus mediated phase in pusa bold ,which is a high yielding variety of Indian mustard widely grown in India.

Agricultural, Brassica, Cotyledons, Regeneration, Shoots, phenotype.

"Standardization of In vitro indirect shoot regeneration protocol for Indian mustard, Brassica juncea(L) using cytokinin, Thiodiazuron as an inducer.", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.6, Issue 6, page no.370-377, June 2019, Available :http://www.jetir.org/papers/JETIR1906H31.pdf

"Standardization of In vitro indirect shoot regeneration protocol for Indian mustard, Brassica juncea(L) using cytokinin, Thiodiazuron as an inducer.", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.6, Issue 6, page no. pp370-377, June 2019, Available at : http://www.jetir.org/papers/JETIR1906H31.pdf