• Sujatha

The present study shows that Chickpea plants under water stress, unstressed plants treated with exogenous EBL and SA exhibited the significant increase in nodule fresh mass and dry mass by over the normal control plants. Whereas co-application of EBL and SA was more significantly increased the nodule fresh mass and dry mass by 23% (0.0281, p≤0.05) and 33.3% (0.0391, p≤0.05) compared with control than their respective individuals. Co-application of EBL and SA was showed the considerable effect on N2ase activity (16%; p=0.0412) than their individual applications over unstressed plants. Co-application of EBL and SA was more effective (28.2%; 0.0265, p≤0.05) over unstressed control than their individual applications in enhancing NR activity in control plants. About 48.3% (0.0187, p≤0.05) of root NiR activity was recorded in plants supplemented with EBL+ SA, suggesting that co-application of EBL and SA has a more significant effect than their individual applications in comparison to control. Plants treated with EBL+ SA showed a marked increase in GS activity (18%; 0.0471 p≤0.05) over unstressed control reflecting the combined effect on modulation of GS activity than their individual applications. Unstressed plants treated with exogenous EBL and EBL+SA exhibited the significant increase in GOGAT activity by 17% and 24.6% respectively over the normal control plants. SA application was able induce the GOGAT activity marginally over the control plants. Exogenous EBL and SA alone and their combination application to unstressed plants exhibited small enhancement in NADH-GDH activity but not statistically significant over the control. Exogenous SA alone application was found to be marginally enhanced the NO3- and NO2- accumulation over the control plants. On the other hand, when plants treated with EBL+SA showed a significant accumulation of NO3- (28.4; 0.0256 p≤0.05) and NO2- (36%; 0.0189 p≤0.05) levels than their individual application compared to normal control. Co-application of EBL and SA was more effective (28.2%; 0.0265, p≤0.05) over unstressed control than their individual applications in enhancing NR activity in control plants. Nitrogen metabolism was reduced by effecting enzymes associated with it. But 28-epibassinolide and salycilic acid application increased nitrogen metabolism even under stress condition. Exogenous application of EBL and SA promotes the growth and development of chickpea plants under different stress conditions. Further research is required for the detailed analysis.

Chickpea, 28-Epibrassinolide, Nitrogen Metabolism, Water stress

"Impact of 24-Epibrassinolide and Salicylic Acid on Nitrogen Metabolism in Chickpea Subjected to Water Stress", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.6, Issue 6, page no.519-540, June 2019, Available :http://www.jetir.org/papers/JETIR1906K63.pdf

"Impact of 24-Epibrassinolide and Salicylic Acid on Nitrogen Metabolism in Chickpea Subjected to Water Stress", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.6, Issue 6, page no. pp519-540, June 2019, Available at : http://www.jetir.org/papers/JETIR1906K63.pdf