• Poovazhagi R

Cellulose is a long chain polymer, made up of rehashing units of glucose, a simple sugar, combined with β-1, 4 glycosidic linkages. Cellulases influence hydrolysis of the individual cellulose strands to break it into little sugars units and at last creating glucose particles. Alkaline Cellulase producing, Bacillus subtilis P15 was isolated from banana orchard soil from Kerala. Among the 27 isolates, 8 strains appearing cellulase activity. From this Bacillus subtilis P15 potential for right down to earth utilizes from on carboxymethyl cellulose (CMC) agar plates and distinguished as Bacillus subtilis P15 strain by morphological, physiological, and biochemical portrayal and 16S rRNA quality investigation. The impact of various parameters like substrate concentration (1.0%), temperature (37⁰), pH (8.0), and incubation time (48 Hrs) was checked with the chosen strain for cellulase production. The purification of cellulase was completed by ammonium sulfate precipitation and dialysis. The molecular weight of the purified carboxymethyl cellulase (CMCase) changed into evaluated to be about 44 kDa with the examination of SDS-page observed by means of staining with Coomassie Brilliant Blue (CBB R-250) providing a simple staining approach and excessive quantitation.

Key Words: Alkaline Cellulase, Bacillus subtilis P15, 16s rRNA, SDS-Page, Enzyme characterization.

"Isolation and Characterization of Alkaline cellulase by Bacillus subtilis P15 from soil", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.6, Issue 6, page no.304-311, June 2019, Available :http://www.jetir.org/papers/JETIR1906L46.pdf

"Isolation and Characterization of Alkaline cellulase by Bacillus subtilis P15 from soil", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.6, Issue 6, page no. pp304-311, June 2019, Available at : http://www.jetir.org/papers/JETIR1906L46.pdf