• Addisu Tamir

The traditionally use of plant materials for the treatment of various diseases is very common in Ethiopia. Dovyalis abyssinica is one of these plants. This plant is native to Africa. The aim of this study was to determine the phytochemical composition and antibacterial activity of methanol extract of leaves of Dovyalis abyssinica. The methanol extract was prepared by maceration and the fractions were obtained by successive fractionation of the methanol extract with n-hexane, dichloromethane, n-butanol and followed by distilled water. The crude methanol leaves extract revealed the presence of phytochemical screening such as flavonoids, alkaloids, saponins, tannins, phenolic, anthraquinones, quinones and terpenoids. Crude methanol and fractionates extract of the leaves were screened for anti bacterial activity against in gram positive bacterial (Staphylococcus aurous and Enterococcus faecalis) and gram negative (Escherichia coli) species using agar well diffusion method at concentrations of 500, 1000 and 10,000 µg/mL. Crude methanol extract exhibited greater activity against gram positive bacteria while n-butanol fraction revealed greater activity against gram negative bacteria. Antibacterial effect of plant extracts might due to the presence of concentrated bioactive compounds or due to synergistic activity of two or more active metabolites. This finding provides a scientific evidence for claimed traditional use of Dovyalis abyssinica.

Phytochemical, Antibacterial activity, Dovyails abyssinica, Agar well diffusion, Escherichia coli, Staphylococcus aurous, Enterococcus faecalis

"PHYTOCHEMICAL SCREENING AND ANTIBACTERIAL ACTIVITY OF LEAF EXTRACTS OF DOVYALIS ABYSSINICA ", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.6, Issue 6, page no.453-465, June 2019, Available :http://www.jetir.org/papers/JETIR1906O67.pdf

"PHYTOCHEMICAL SCREENING AND ANTIBACTERIAL ACTIVITY OF LEAF EXTRACTS OF DOVYALIS ABYSSINICA ", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.6, Issue 6, page no. pp453-465, June 2019, Available at : http://www.jetir.org/papers/JETIR1906O67.pdf