• Kalyani R Gunjal
• Dr A.D. More

Phaseolus lunatus (L.) belongs to family Fabaceae. Lima beans, like many other legumes, which are a good source of dietary fibre, and a virtually fat free source of high – quality protein. The most abundant mineral in the raw lima bean is potassium, followed by calcium, phosphorous, magnesium, sodium, and iron. When lima beans germinated, there was increased calcium and phosphorus. Additionally, it is a good source of vitamin B-6. Lima bean is commonly grown for its edible seeds. It is one of the more important vegetable legume in the United States. Lima bean is a perennial herb grown as an annual. Leaves have three leaflets. Seeds and leaves are said to have astringent properties, the vines are used as cattle feed, the young leaves are used to prepare hay, and immature dried seeds can be made into flour. The green beans contain 1.3g protein, green seeds;8.4g and green leaves;0.6g per 100g edible portion. Seeds contain cyanogenic glucoside linamarin which may cause poisoning when eaten raw. The cultivar of lima bean, ‘King of Garden’(Pole) variety seeds were treated with chemical mutagen Ethyl Methenesulphate (EMS) at the concentration of 0.25%, 0.50%, 0.75%, 1% and physical mutagen Gamma rays at the dosage of 240Gy, 300Gy, 360Gy, 420Gy and also with combination of both (Gamma rays and EMS) like 240Gy+1%, 300Gy+0.75%, 360Gy+0.50%, 420Gy+0.25%. Mutagens play an important role to assess the effect of mutagenic treatments. In present investigation all the mutagenic treatments were successful in inducing mutagens in lima beans. In M1 biological parameters like seed germination percentage, seedling height and seedling injury were studied. These biological parameters decreased with increases in different mutagenic treatments which were recorded. Seedling height and seedling injury was decreased with increases in different mutagenic concentration and doses of EMS and Gamma rays.

EMS, Gamma rays, lima bean, seedling height and seedling injury.

"STUDY OF M1 BIOLOGICAL PARAMETERS IN LIMA BEAN (Phaseolus lunatus L.) INDUCED BY CHEMICAL AND PHYSICAL MUTAGENS", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.6, Issue 6, page no.555-566, June 2019, Available :http://www.jetir.org/papers/JETIR1906P80.pdf

"STUDY OF M1 BIOLOGICAL PARAMETERS IN LIMA BEAN (Phaseolus lunatus L.) INDUCED BY CHEMICAL AND PHYSICAL MUTAGENS", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.6, Issue 6, page no. pp555-566, June 2019, Available at : http://www.jetir.org/papers/JETIR1906P80.pdf