• Kamble Sonali R
• Dr. A. D. More

Vicia faba, L. also known as the Broad bean, It belongs to family Fabaceae. Mutation breeding is the potential tool for creating variability. It is modern and useful tool employed by mutation breeders with help of physical and chemical mutagens like different doses/ concentrations of Gamma rays, EMS and NMU were used by several workers. Induced mutation breeding is one of the most efficient and modern scientific techniques. Inducing desirable mutations and exploiting them for crop improvement is known as Mutation breeding. The induced mutation through physical and chemical mutagen is very effective tool to induce variations for significant characters. Broad bean is rich in Carbohydrates, Proteins, Vitamins (Riboflavin, niacin), Fats, Minerals and Fibres. The present research work was conducted to study morphological changes and chlorophyll mutation in leaves of Vicia faba by single and combined treatment of Gamma rays and Ethyl Methanesulphonate. Screening of mutation was carried out in M1 generation of Vicia faba variety Wal Kokan Bhushan. The seeds were given treatments were given to individual. Doses/concentrations of gamma rays (240 Gy, 300 Gy, 360 Gy, 420 Gy) and EMS (0.25%, 0.50%, 0.75%, 1%), and combination of Gamma rays and EMS (240Gy+0.25%, 300Gy+0.50%, 360 Gy+0.75%, 420 Gy+1% EMS). Different types of chlorophyll mutation were observed like xantha, viridis, aurea, maculate. The frequency of chlorophyll mutation was increased with decrease in doses/concentrations of both single and combined treatments. Also two types of flower colour mutations were observed and recorded like light yellow and creamish white colour.

Ethyl Methanesulphonate, Gamma rays, Xantha, Viridis, Maculate, Chlorophyll mutation, NMU

"Induction of Mutations in Vicia faba L. through Physical and Chemical Mutagens. ", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.6, Issue 6, page no.93-100, June 2019, Available :http://www.jetir.org/papers/JETIR1906Q13.pdf

"Induction of Mutations in Vicia faba L. through Physical and Chemical Mutagens. ", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.6, Issue 6, page no. pp93-100, June 2019, Available at : http://www.jetir.org/papers/JETIR1906Q13.pdf