Abstract
In this study, phytochemical screening was carried out to find the presence of the active chemical constituents in the solvent extract (ethanol, ethyl acetate, chloroform, methanol and acetone) of Aerva lanata. The phytochemicals such as, flavonoids, alkaloids, phenolic compounds, tannins, steroids, saponins, terpenoids and glycosides were studied. The leaf of A. lanata was extracted with various solvents and antimicrobial activity was studied. The leaf extract showed moderate activity against E. coli, S. epidermis, E. aerogenes and B. cereus. The leaf extract of A. lanata showed 9 mm zone (chloroform extract), 11 mm zone (methanol extract) and 13 mm zone (acetone extract) against E. coli. The zone of inhibition was 11 mm (ethanol extract), 10 mm (ethyl acetate extract), 11 mm (chloroform extract), 10 mm (methanol extract) and 13 mm (acetone extract) against S. epidermis. The leaf of A. lanata was effective against E. aerogenes. The zone of inhibition was 9 mm in ethanol and ethyl acetate extract, 10 mm in methanol extract and 11 mm in acetone extract. The chloroform extract of leaf showed moderate activity (9 mm zone) against B. cereus, however, other extracts did not show any activity. The MIC concentrations ranged from 40 mg/ml to 80 mg/ml. The methanolic extract of A. lanata was highly effective against E. aerogenes and the MIC was 40 mg/ml. The result of present finding showed the presence of alcohol, alkenes, aromatic, amine, phenyl, ether, methylene, primary amines, 1° and 2° amine and aliphatic chloro compound. The peaks were observed at 3308.3, 2853.3, 2701.6, 1660.75, 1500.2, 1385.8, 1225.05, 1160.75, 1096.45, 982.25, 821.5, 757.2 and 660.7 cm-1. The major band was observed at 3308 cm-1 that mainly could be O-H stretching vibrations of alcohol group.