• CHAUDHARY ARPIT RAMESHBHAI

Tebuconazole is a Triazole fungicide used agriculturally to treat plan pathogenic fungi. It is widely use for apple and grapes. Technical grade of tebuconazole was collected from shufalan crop science. Stock solution of 2500 ppm made by acetone, tebuconazole and pulp of apple. Test solution of 500 ppm, 1500 ppm, 2500 ppm, 3500 ppm made for analysis in SHIMADZU GC-MS QP 2010 ULTRA equipped with single quadrupole mass detector. 1 micro litre of sample used in every analysis. In linearity test five concentration sample were injected and the graph of area versus concentration found linear. The sample of 2500 ppm which was injected five times for precision study the area (12648071) and retention time (RT-9.38) found same for individual injection. Taken the 500 ppm, 2500 ppm, and 4500 ppm of sample set as three sample of each for accuracy test and in the result the average of area and retention time were accurate for individual sample set. Tebuconazole is highly toxic for human body therefore FSSAI decided maximum residue limit 1 ppm. The method of GC-MS developed to find out the quantity of tebuconazole present in apple. The limit of quantification of the method was 30 ppm and the limit of detection was 10 ppm.

GC-MS, tebuconazole, fungicide.

"ANALYTICAL METHOD DEVELOPMENT, VALIDATION AND OPTIMIZATION FOR THE DETERMINATION OF TRIAZOLE FUNGICIDES BY GAS CHROMATOGRAPHY MASS SPECTROMETRY", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.6, Issue 6, page no.307-313, June-2019, Available :http://www.jetir.org/papers/JETIR1907042.pdf

"ANALYTICAL METHOD DEVELOPMENT, VALIDATION AND OPTIMIZATION FOR THE DETERMINATION OF TRIAZOLE FUNGICIDES BY GAS CHROMATOGRAPHY MASS SPECTROMETRY", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.6, Issue 6, page no. pp307-313, June-2019, Available at : http://www.jetir.org/papers/JETIR1907042.pdf