• KEERTHIKA REDDY SINGALREDDY
• Dr.B.RAVI SANKAR
• SARANYA KALAI

Hormones produced by the adrenal glands and adipose tissues have important roles in normal and physiology and are altered in many disease states.Studies have shown that obesity is associated with changes in adrenal function ,including increase in adrenal medullary catecholamine output ,alterations of the hypothalamic-pituitary adrenal (HPA) axis,elevations in circulating aldosterone together with changes in adipose tissue glucocorticoid metabolism,and enhanced adipocyte mineralocorticoid receptor activity. However ,the impact of obesity on adrenal structure and the mechanism behind the altered adrenal function is not yet studied. The main objective is to study the effect of cafeteria diet-induced obesity on the adrenal steroidogenic machinery. Sprague-Dawley female rats of 21 days old ,weighing 30-35 grams were taken. The rats were divided into two groups,with 6 animals. Group-1 rats are the controlled rats which were fed with standard chow for 20 weeks and the Group-2 rats are the cafeteria diet fed rats which included standard chow along with random selection of highly energetic and palatable human foods like pork,chicken,cakes ,cookies ,chocolate ,marsh mallow and cheese. During the study period ,food consumption ,body weight and estrous cyclicity were recorded daily.Rats were killed between PND160 and 170 at diestrus phase.Abdominal girth ,and overall fat were weighed,and then adrenals were dissected out and stored at -20 degree. StAR ,IR(Insulin receptor) and MCR (Mineralocorticoid receptor) expressions in rat adrenal tissue were assessed by western blotting. The final result is that the StAR protein expression and the Mineralocorticoid receptor protein expression were significantly decreased and the Insulin receptor protein expression was significantly increased in the adrenal of cafeteria diet-induced obese rats compared to control rats.

Hypothalamic-pituitary adrenal (HPA) axis ,mineralocorticoid receptor , steroidogenic machinery , diestrus phase , StAR protein , insulin receptor protein

"IMPACT OF CAFETERIA DIET-INDUCED OBESITY ON THE ADRENAL OF ADULT FEMALE RATS ", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.6, Issue 6, page no.353-364, June-2019, Available :http://www.jetir.org/papers/JETIR1907048.pdf

"IMPACT OF CAFETERIA DIET-INDUCED OBESITY ON THE ADRENAL OF ADULT FEMALE RATS ", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.6, Issue 6, page no. pp353-364, June-2019, Available at : http://www.jetir.org/papers/JETIR1907048.pdf