• RAHUL GUPTA
• Dr. Kadirvelu Jeevaratnam

The current in vitro exploration discovered the phytochemical components in MeOH extracts of bark (GGB) and leaf (GGL) parts of Galphimia glauca which were found to be effective in scavenging free radicals, obstructing the growth of MRSA, subduing the cancer cell proliferation (A549 and SW480) and displaying less toxicity towards the normal cell line (HEK293). The total flavonoids content (TFC) of GGB and GGL were 21.6 ± 0.34 and 20 ± 0.26 µg QE/ml, while total phenolic content (TPC) were 26.6 ± 0.5 and 21.8 ± 0.33 µg GAE/ml, respectively. However, the bark extract (GGB) showed elevated level of TPC and TFC than the leaf extract (GGL) and the same pattern was discovered in the evaluation of reducing power (FRP and FRAP) and total antioxidant activity (TAA). In DPPH assay, IC50 value of the extracts GGB and GGL were 43.7 ± 0.2 µg/ml and 48.8 ± 0.1 µg/ml respectively, while, IC50 value of quercetin was 25.2 ± 0.15 µg/ml but both the extracts exhibited slightly lower IC50 value in contrast to quercetin. The spectrum of antibacterial action of both the extract (GGB and GGL) was determined against the clinical and standard (ATCC 33591) methicillin-resistant Staphylococcus aureus (MRSA) through Agar well-diffusion assay by quantifying the Zone of Inhibition (ZI). ZI value for the extracts - GGB and GGL against the clinical MRSA strain was 16 ± 2 mm and 15 ± 1.5 mm respectively, while against the standard (ATCC 33591) MRSA strain was 15.3 ± 0.57 mm and 14 ± 1 mm respectively, at 10 mg/ml. Further, to evaluate and calculate IC50 value for anti-proliferative and cytotoxic effects of G. glauca plant extracts (GGB and GGL), MTT assay was utilized. The IC50 value for the GGB against A549, SW480, and HEK293 at 24 hours was 157.8 ± 2.44 µg/ml, 136.6 ± 2.73 µg/ml, and 388.67 ± 6 µg/ml, respectively, while IC50 value for the GGL against A549, SW480, and HEK293 at 24 hours was 194 ± 4.64 µg/ml 178 ± 3.1 µg/ml, and 317.2 ± 9.4 µg/ml, respectively. Hence, the extract of bark (GGB) and leaf (GGL) of G. glauca can be exercised as a therapeutic remedy against the detrimental diseases and disorders triggered by oxidative stress, an allergic reaction, and pathogenic invasion.

Galphimia glauca, Antioxidant activity, Antibacterial, Methicillin-Resistant Staphylococcus aureus (MRSA), Anticancer

"A Comparative Evaluation of In Vitro Phytochemical Analysis, Antioxidant, Antibacterial and Anticancer Activity of Methanolic (MeOH) Crude Extract of Bark (GGB) and Leaf (GGL) of Galphimia glauca ", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.6, Issue 6, page no.830-842, June-2019, Available :http://www.jetir.org/papers/JETIR1907115.pdf

"A Comparative Evaluation of In Vitro Phytochemical Analysis, Antioxidant, Antibacterial and Anticancer Activity of Methanolic (MeOH) Crude Extract of Bark (GGB) and Leaf (GGL) of Galphimia glauca ", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.6, Issue 6, page no. pp830-842, June-2019, Available at : http://www.jetir.org/papers/JETIR1907115.pdf