• Dr.S.USHA
• Dr.S.KARPAGAM
• Dr.N.SENTHILKUMAR
• -
• -

ABSTRACT The present study was performed to investigate the antioxidant activity, total phenol, terpenoids content and anticancer activity from the leaf of mother plant, in vitro leaf and callus extract of Eupatorium triplinerve. The leaf and callus extracts were evaluated for antioxidant activities by DPPH (1, 1– diphenyl -2- picryl - hydrazyl) radical scavenging assay. Among three different extracts with ethanol solvent used, maximum antioxidant activity was found in ethanol callus extract (19.76%) from Azhiyar accession followed by others. Total phenol and terpenoids content was quantitatively estimated which recorded maximum in Azhiyar accession 16.23 mg Gallic Acid Equivalents (GAE)/g and 8.36 mg Quercetin Equivalents (QE)/g). In vitro anticancer activity was evaluated using MTT assay at different concentrations. The maximum callus extract was found to be 75% at a dose of 250mg /ml. From the results, it is concluded that, the ethanol callus extract of Eupatorium triplinerve exhibited high level of In vitro anticancer activity, which may be due to the presence of phenol, Terpenoids and related polyphenols.

Keywords: Eupatorium triplinerve, antioxidant activity, DPPH, phenol and terpenoid.

"Studies on Antioxidant and Anticancer Activity from Leaf and Callus Extract of Eupatorium triplinerve vahl", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.6, Issue 6, page no.932-940, June 2019, Available :http://www.jetir.org/papers/JETIR1907129.pdf

"Studies on Antioxidant and Anticancer Activity from Leaf and Callus Extract of Eupatorium triplinerve vahl", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.6, Issue 6, page no. pp932-940, June 2019, Available at : http://www.jetir.org/papers/JETIR1907129.pdf