• D.Praveen
• R.v.valli kumari

A simple, rapid, precise, sensitive and reproducible reverse phase high performance liquid chromatography (RP-HPLC) method has been developed for the quantitative analysis of Cefixime and Lactobacillus in pharmaceutical dosage form. Chromatographic separation of Cefixime and Lactobacillus was achieved on Waters Alliance -2695, by using Symmetry shield RP 18, 150x4.6mm, 5 µm column and the mobile phase containing 3.0gm Octane-1-Sulphonic acid is dissolved in 1lt water adjust pH-2.5 with OPA & ACN in the ratio of 60:40% v/v. The flow rate was 1.0 ml/min, detection was carried out by absorption at 282 nm using a photodiode array detector at ambient temperature. The number of theoretical plates and tailing factor for Cefixime and Lactobacillus were NLT 2000 and should not more than 2 respectively. % Relative standard deviation of peak areas of all measurements always less than 2.0. The proposed method was validated according to ICH guidelines. The method was found to be simple, economical, suitable, precise, accurate & robust method for quantitative analysis of Cefixime and Lactobacillus and study of its stability.

HPLC, Cefixime and Lactobacillus.

"simultaneous method development and validation of cefixime and lactobacillus by using RP-HPLC ", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.6, Issue 6, page no.694-704, June 2019, Available :http://www.jetir.org/papers/JETIR1907244.pdf

"simultaneous method development and validation of cefixime and lactobacillus by using RP-HPLC ", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.6, Issue 6, page no. pp694-704, June 2019, Available at : http://www.jetir.org/papers/JETIR1907244.pdf