• Ananthi.V.
• Selvakumar.G.
• Arun.A.

Increasing cost of petroleum based fuel along with its impact throughout the world are main objectives in growth and development of alternate renewable energy resources. Among the liquid fuels, biodiesel is the most promising source since it can be used with the present engines without any modification of the engine design. Microalgae is considered as the fruitful energy source of biodiesel nowadays. Inorder to improve the growth rate, biomass production and lipid accumulation, heterotrophic mode of cultivation is highly preferred. Hence the present study compared the impacts of both autotrophic and heterotrophic mode of cultivation towards microalgal growth. From the fresh water three oleaginous microalgal strains (Chlorella sp., Spirulina sp., Scenedesmus sp.) were screened and chosen for further analysis. While comparing both environmental conditions(Autotrophic and heterotrophic) for biodiesel production using fresh water microalgae maximum biomass(2.52±0.015 g/L) was attained by the microalgal strains on culturing under heterotrophic condition. While analyzing the lipid content of microalgal strains no significant difference was found on the two mode of cultivation.

Microalgae, Autotrophic, Heterotrophic, FAME, Biodiesel.

"Third generation biofuel production by using freshwater microalgae", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.6, Issue 6, page no.857-864, June 2019, Available :http://www.jetir.org/papers/JETIR1907427.pdf

"Third generation biofuel production by using freshwater microalgae", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.6, Issue 6, page no. pp857-864, June 2019, Available at : http://www.jetir.org/papers/JETIR1907427.pdf