• Dr.Osama Asanousi Lamma
• Swamy A.V.V.S
• Amna Ali Mohamed Alhadad

Laccase is a copper-containing polyphenol oxidase that acts on a wide range of substrates. This enzyme is found in many plant species and is widely distributed in fungi including wood-rotting fungi where it is often associated with lignin peroxidase, manganese dependent peroxidase, or both. Laccases catalyze the polymerization of several phenolic substances to polymeric products. In addition they transform lignin and lignin -related compounds, showing very broad substrate specificity. Laccases are used in a variety of applications, such as bioremediation, bioleaching of denims in processing of beverages, as bio analytical tool in biosensors to estimate the quantity of phenols in natural juices or the presence of other enzymes. Recent success in fungal molecular and cellular engineering technology has contributed to significantly increase the industrial production of recombinant laccase. Kinetic (Michaelis -Menten parameters, optimum pH, k cat) and stability properties of Laccases vary according to the source of the enzymes. Exploration of microbial diversity for novel Laccases can yield improved Laccases for varied application. This work presents primary investigations on the isolation and Purification of Laccases from different fungal strains.

Polymerization, lignin, Recombinant laccase

"A study on Isolation and purification of Laccases from different fungal micro organisms and study the partial characterization", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.6, Issue 6, page no.371-376, June 2019, Available :http://www.jetir.org/papers/JETIR1907652.pdf

"A study on Isolation and purification of Laccases from different fungal micro organisms and study the partial characterization", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.6, Issue 6, page no. pp371-376, June 2019, Available at : http://www.jetir.org/papers/JETIR1907652.pdf