• Sonwane P A
• Manvar A V

Abstract : Among 24 LAB isolates from 32 various dairy samples, seven strains were screened by lipolytic activity using tributyrine agar plates. All seven selected LAB isolates possesses lipolytic activity are characterized by Biochemical and morphological tests identified the character resemblance to Lactobacillus sp. These isolates are designated as L13, L14, L15, L16, L17, L18, and L19. All isolates were studied for major four probiotic attributes such as acid tolerance, bile salt tolerance, antibacterial activity and antibiotic resistance. Acid tolerance was performed at pH 3 and pH 4, while bile salt tolerance was performed at 0.15% and 0.3% concentration of bile salt, both tests were assessed by turbidometric growth measurement as optical density at 600 nm. Antibacterial activity was performed by Agar well diffusion against S. aureus, E. Coli, B. cereus and Salmonella typhi. Antibiotic sensitivity was accessed by disc diffusion test using standard antibiotic discs (Hi media) of novobiocin (NV) and erythromycin(E), chlortetracycline(CT), gentamycin, tobramycin(TOB), collistrin(CL), carbenicilin(CB), nitrofurantoin(NIT), lincomycin(L), cefuroxime(CXM), Kanamycin(K). In present study, among seven lipolytic LAB isolate L17 and L18 have good probiotic potential with efficient lipolytic activity, while L15 and L16 have efficient probiotic activity with less lipolytic activity.

LAB, MRS, Probiotic, GI tract, Lipolytic activity

"PROBIOTIC PROPERTIES OF LACTIC ACID BACTERIA HAVING LIPOLYTIC ACTIVITY", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.6, Issue 6, page no.471-477, June 2019, Available :http://www.jetir.org/papers/JETIR1907668.pdf

"PROBIOTIC PROPERTIES OF LACTIC ACID BACTERIA HAVING LIPOLYTIC ACTIVITY", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.6, Issue 6, page no. pp471-477, June 2019, Available at : http://www.jetir.org/papers/JETIR1907668.pdf