• V. Sreeja
• R.V. Valli kumari
• M. Sathish kumar
• S. Marakatham

ABSTRACT A analytical method employing reverse phase high performance liquid chromatography with PDA detection was developed to assay meropenem and vaborbactam in bulk and injection dosage form. The assay of meropenem and vaborbactam was achieved by Supelco C8 column (25 cm × 4.6 mm, 5 μm particle size) with isocratic elution. The mobile phase involved is methanol and potassium dihydrogen phosphate (0.1 Molar) mixed in ratio 65/35 (v/v). The eluted meropenem and vaborbactam are monitored at wavelength 235 nm. The procedure employed separated meropenem and vaborbactam within six min run time. Retention time of meropenem and vaborbactam were found to be 2.915 and 3.406. The parameters were tested by validating system suitability, linearity, sensitivity, selectivity, accuracy, robustness and precision. Linearity of meropenem and vaborbactam was in range of concentration 500 – 1500 µg/ml. The LOD and LOQ values were found to be 0.706 and 2.352 µg/ml for meropenem and 0.453 and 1.510 µg/ml for vaborbactam. For precision, relative standard deviation were found to be 0.102% (meropenem) and 0.050% (vaborbactam). The percent recoveries were 100.20 -100.36% and 99.60-99.67% for meropenem and vaborbactam respectively. The selectivity results proved the non interference from excipients. So this method is recommended for the routine analysis of meropenem and vaborbactam in laboratories of quality control.

Key words: Meropenem, Vaborbactam, RP-HPLC Method, Validation.

"Development and Validation of an RP-HPLC Method for Simultaneous Estimation of Meropenem and Vaborbactam in Bulk and Dosage Form", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.6, Issue 6, page no.934-942, June 2019, Available :http://www.jetir.org/papers/JETIR1907A13.pdf

"Development and Validation of an RP-HPLC Method for Simultaneous Estimation of Meropenem and Vaborbactam in Bulk and Dosage Form", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.6, Issue 6, page no. pp934-942, June 2019, Available at : http://www.jetir.org/papers/JETIR1907A13.pdf