• Souvagyalaxmi Sahoo
• Stuti Sinha
• Kausal Sharma
• Sabiha kaifi

Abstarct: To establish a standardized protocol for in vitro propagation of endangered plant Bryophyllum pinnatum using leaf explants, for long term conservation with its quantitative, quantitative screening and thin layer chromatography analysis to make the plant commercially available. The highest rate of shoot proliferation (85%) and number of shoots per explant 10 was obtained on media BAP 2.5mg/l + Kn0.5mg/l with average shoot length 2.0±0.66. The maximum shoot regeneration showed 80±1.45 in BAP 1.5mg/l + NAA 0.5mg/l + IBA 0.5mg/l .The largest effect on root formation occurred in BAP 0.5mg/l+ IBA 0.5mg/l +NAA 2.0 mg/l and 45±0.0 percentage of rooted explants with 9±11.5 number of roots produced. The plants were maintained at about 70% relative humidity in the greenhouse with 75% shading with 98% of survival rate was achieved after 6 weeks. The phytochemical screening of the Ethanolic and Aqueous leaf extracts of Bryophyllum pinnatum showed the presence of alkaloids, flavonoids, carbohydrates, saponins, terpinoids, oxalate, streroids, tannins, glycosides, protein and phenolic compounds with presence of rich amount of chlorophyll (0.7236µg/ml), Free amino acid (234.96µg/ml), protein (24.402µg/ml) and carbohydrate (513.90µg/ml). Thin layer chromatography showed the presence of amino acids (0.77cm) and cardiac glycosides (0.88cm) from leaf extracts.

Micropropagation, Growth regulators, Ethanolic and Aqueous leaf extracts, Phytochemical screening, Thin layer chromatography.

"Micropropagation of endengered plant Bryophillium pinatum with its quantitative screening and thin layer chromatography", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.6, Issue 6, page no.12-22, June 2019, Available :http://www.jetir.org/papers/JETIR1907D03.pdf

"Micropropagation of endengered plant Bryophillium pinatum with its quantitative screening and thin layer chromatography", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.6, Issue 6, page no. pp12-22, June 2019, Available at : http://www.jetir.org/papers/JETIR1907D03.pdf