• Kirubha P
• Sakthi Shree K

The chief object of this study was to determine the efficacy of Anonna muricata leaf extract in alleviation of cypermethrin induced testicular histology in albino rats. Wistar strain albino rats of 130-250 gms body weight (BW) were divided into six groups of five rats. Group I served as normal control. Group II given cypermethrin (CYP) (0.05 ml/100g BW/day) for 60 days and Group III given aqueous Anonna muricata leaf extract (AMLE) 0.05 ml/100g BW/day) for 60 days orally, while Group IVwas treated with Methotrexate (MTX) (1.0mg/100g BW/day) once a week intraperitoneally. Group V and Group IV given cypermethrin (0.05 ml), with supplementations of A. Muricata leaf (0.05 ml) and methotrexate (1.0 mg /100 gm BW/week) resp. for 60 days. At the end of experimental period all the animals were sacrificed and analysed for testis histology. On comparison with control group, there was no significant increase in BW of any of the treatment groups, except for a significant decrease in (MTX) alone given group. When compared to CYP treatment, no significant decrease in BW can be seen in both the AMLE as well as MTX supplemented groups. When compared to control, a significant decrease in weight of testis can be observed in all the treatment groups with the decrease being less in the CYP treated MTX supplemented group, On comparison with CYP treatment no significant change in the weight of testis is observed on the treatment with either AMLE of MTX alone or in AMLE supplemented group, but MTX supplementation seems to be effective in increasing the testis weight to a certain extent. CYP treated testis showed degenerative changes and vacuolation in germ cell layers with basement membrane damage and interstitial edema with atrophy of Leydig cells. AMLE treated testis showed seminiferous tubules with spermatogonia on basement membrane and Leydig cells in the interstitial region. MTX treated testis showed regular tubules with degenerating cells separating from basement membrane and rupture in certain regions along with lymphocyte infiltration. AMLE and CYP treated testis showed testicular damage being rectified by appearance of seminiferous tubules enclosing most of the stages of germ cells, and spermatids in the lumen, with slight vacuolations. CYP treated MTX supplemented testis showed rectification of damage to seminiferous tubules part of the germinal stages, with disruption basement membrane and lymphocyte infiltration in interstitial spaces.

Albino Rats, Anonna Muricata Leaf extract, Cypermethrin, Methotrexate, and testicular histology.

"Efficacy Of Anonna Muricata Leaf Extract In Alleviation Of Cypermethrin Induced Testicular Toxicity In Albino Rats", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.6, Issue 6, page no.987-997, June 2019, Available :http://www.jetir.org/papers/JETIR1907G37.pdf

"Efficacy Of Anonna Muricata Leaf Extract In Alleviation Of Cypermethrin Induced Testicular Toxicity In Albino Rats", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.6, Issue 6, page no. pp987-997, June 2019, Available at : http://www.jetir.org/papers/JETIR1907G37.pdf