• Pratibha Parihar

The withanolides including withaferin A and withanolide A and D present in the root and leaf extracts of Withania somnifera (L.) Dunal were analyzed by TLC with solvent system chloroform: methanol (9:1) showed clear and well resolved spots using methanolic leaf and root extract of Ashwagandha. The results confirmed the presence of high concentration of withaferin A in the leaf and withanolide A in the root with Rf value of 0.32 and 0.48, respectively. Quantitative and qualitative analysis was further performed by HPLC with a solvent system ethanol: water (1:1) and result showed that mycorrhiza inoculation increased the total withanolide production by 42% in leaf and 51% in the root. Thus, AM symbiosis can significantly increase the content of some secondary metabolites in Ashwagandha.

Withania somnifera, withaferin A, withanolide A, HPLC

"Influence of mycorrhizal infection on withaferin A and withanolide A & D production in root and leaf of Withania somnifera (L.) Dunal", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.6, Issue 6, page no.607-616, June 2019, Available :http://www.jetir.org/papers/JETIR1907I75.pdf

"Influence of mycorrhizal infection on withaferin A and withanolide A & D production in root and leaf of Withania somnifera (L.) Dunal", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.6, Issue 6, page no. pp607-616, June 2019, Available at : http://www.jetir.org/papers/JETIR1907I75.pdf