• V.V.Nimbalkar
• Singar Anjali Sharad
• Joshi Kajal Dhiraj

ABSTRACT Objectives: To evaluate in vitro antioxidant and anticataract activity of Diosgenin against glucose induced cataractogenesisusing goat lenses. Materials and Methods:Transparent isolated goat lenses were incubated in artificial aqueous humor and divided into five experimental groups. The Diosgenin at a dose of 20µg/ml is incubated simultaneously with glucose(55mM)and glucose (5.5mM) for a period of 72 hours ,Ascorbic acid (20µg/ml)is used as the standard drug.At the end of incubation lens opacity is measured by photographic evaluation. Antidenaturing activity is evaluated by taking different concentration of Diosgenin (10p.p.m-500p.p.m)with egg albumin and subjected for protein denaturation by using heat for 15 min. Standard drug used is Diclofenac sodium,at the end protein denaturation is analysed by taking absorbance at 440nm and 660nm. Results:Diosgenin exhibited concentration dependent inhibition of thermally induced protein denaturation and also shows significant inhibition of cataractogenesis of eye lenses by Diosgenin at conc. 20 p.p.m. Conclusion:The present study supports Diosgenin as an antidenaturing and anticataract agent.

Cataract, Ascorbic Acid,Diosgenin,Antioxidants,Antidenaturation

"Correlating antidenaturation and anticataract : A novel approach of treating cataract ", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.6, Issue 6, page no.126-132, June 2019, Available :http://www.jetir.org/papers/JETIR1907J20.pdf

"Correlating antidenaturation and anticataract : A novel approach of treating cataract ", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.6, Issue 6, page no. pp126-132, June 2019, Available at : http://www.jetir.org/papers/JETIR1907J20.pdf