• Dr. Venkatesh Vamanrao Kulkarni
• Shravani Parandekar
• Riyaa Ranade

Staining of tissue specimens using gold standard haematoxylin and eosin stain enables the evaluation of the morphology of cell but not the protein activity. Phosphotungstic Acid-Haematoxylin Stain (PTAH Stain) demonstrates the protein activity within the cells as cells with higher protein activity take up more stain. Higher the protein activity, faster is the transformation of a normal cell into a malignant cell. Hence, this study has been taken up to evaluate the protein activity within the cell which helps in early diagnosis, treatment plan and prognosis of the lesion. In mild and moderate dysplasia cases, PTAH stain have found ability in exploring undermined high protein activity in initial dysplastic cases. So, PTAH stain can be used as a potential marker for protein activity and transformation of mild to moderate dysplasia, moderate to severe dysplasia and severe to malignant dysplastic transformation of cell.

PTAH, protein activity, dysplasia

"Evaluation of protein activity in different grades of oral dysplasia using Phosphotungstic acid- haematoxylin stain (PTAH stain) –A Pilot Study", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.6, Issue 6, page no.452-457, June 2019, Available :http://www.jetir.org/papers/JETIR1907K69.pdf

"Evaluation of protein activity in different grades of oral dysplasia using Phosphotungstic acid- haematoxylin stain (PTAH stain) –A Pilot Study", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.6, Issue 6, page no. pp452-457, June 2019, Available at : http://www.jetir.org/papers/JETIR1907K69.pdf