Abstract
Comparative studies on leaf Anatomy of five Cassia species
V. Selvi1 and S. Karpagam2
1. Assistant Prof.,Dept of Plant Biology and Plant Biotechnology,Meenakshi College for Women,Chennai-24,India
2. Associate Prof., Dept of Botany Queen Mary’s College (Autonomous), Mylapore,
Chennai 600 004, India
Abstract
The plant kingdom has for long time served prolific source of helpful drugs, food, additives, flavouring agents, colorants binders and lubricants etc., as a matter of fact, it was estimated that about 25% of all prescribed medicines today are substances derived from plants. The anatomical study was focused: midrib of the leaf, lamina of the leaflet, epidermal cells and stomata, foliar sclereids and venation Pattern of five species of cassia (C. occidentalis, C. auriculata, C. alata, C. fistula and C. roxburghii). The specimens were sectioned with the help of rotary microtome, dewaxing of the sections and sections were stained with Toluidine blue and Tannic acid-Ferric chloride. For studying the stomatal morphology, venation pattern and trichome distribution, clearing of leaf with sodium hydroxide or epidermal peeling by partial maceration employing Jeffrey’s maceration fluid. There is variation between studied species in abaxial side and the adaxial part of the midrib. There are also differences between epidermal layers, mesophyll tissues and venations of five species. Anatomical data have been used to good effect at all levels of the taxonomic hierarchy, as well as for identification and assessment of the taxonomic relationships among taxa of the flowering plants.