• DEVAMANI, M
• R. S. UMAKANTH

Two races of silkworm namely PM X CSR2 and CSR2 were selected for the present investigation. The digestive juice and haemolymph were collected from 2nd, 4th and 6th day larvae of fifth instar fed with Dimethoate treated leaves in 10, 20 and 30 ppm. The samples were subjected to spectrophotometric analysis for amylase activity and the recorded O.D. values (at 525 nm) were converted into maltose released. Accordingly, the results clearly indicate that the amylase activity gets enhanced with the increase in number of feedings along with the advancement of day wise in 5th instar larva. PM X CSR2 performed better for amylase activity in haemolymph with respect of dimethoate treated mulberry leaf varieties in 30ppm concentration at 12h after treatment, compared to CSR2 and control batch and was also true in 10ppm, 20ppm. But in gut juice of silkworm CSR2 has shown better performance better at 30ppm, at12h after treatment than PM X CSR2 and also in other concentrations used for treatment. Amylase activity was found to be higher in gut juice than haemolymph of silkworm in all the batches understudy.

Bombyx mori L. Amylase, Digestive juice, Haemolymph, Spectrophotometer.

"“Quantitative and qualitative evaluation of effect of Dimethoate on amylase activity in Silkworm Bombyx mori Linnaeus.”", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.6, Issue 6, page no.96-107, June 2019, Available :http://www.jetir.org/papers/JETIR1907N53.pdf

"“Quantitative and qualitative evaluation of effect of Dimethoate on amylase activity in Silkworm Bombyx mori Linnaeus.”", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.6, Issue 6, page no. pp96-107, June 2019, Available at : http://www.jetir.org/papers/JETIR1907N53.pdf