• K. ASHOK KUMAR
• P. PRAGATHI
• JITENDRA PATEL

In current investigation, a popular ayurvedic crude drug Desmostachya bipinnata also known as darbha was studied for in-vitro antioxidant activity. The drug has been identified by HPTLC fingerprint analysis. The dried rhizome and roots were pulverized into fine powder. Required quantity of powder was employed for successive extraction by using soxhlet apparatus. The successive extracts were used for HPTLC fingerprint investigations, while the Hydro-alcoholic extract was prepared separately for antioxidant studies. A silica gel 60F254 TLC precoated aluminium plates (10 x 10 cm, layer thickness 0.2mm, Merck, Mumbai) were employed as a stationary phase. About 1µL of the sample was applied on the TLC plate under a stream of nitrogen using a semiautomatic spotter and the bands were detected employing a deuterium lamp. The chromatographic conditions were optimized and estimations were performed on a stationary phase, pre coated silica gel60 F254 aluminum sheets (10 x10) which were prewashed with the n-hexane (for n-hexane extract), chloroform (for chloroform extract) and methanol (for methanol extract) and dried in air. Then the plated were saturated with the respective mobile phases for 30 minutes and the migration distance allowed was 72mm. The wave length scanning was performed at 254nm for n-hexane extract and 366nm for chloroform and methanol extract. The source of radiation was deuterium lamp emitting a continuous UV spectrum between 190-400nm. The samples were spotted and developed at 25±2°C. The HPTLC finger prints of n-hexane, chloroform and methanol extracts were developed employing following mobile phases respectively. a) Hexane: ethyl acetate: acetic acid (8:2:0.5 v/v) at 254 nm. b) Toluene: ethyl acetate: acetic acid (8:2:0.2 v/v,) at 366 nm. c) Toluene: ethyl acetate: acetic acid (8:2:0.2 v/v) at 366 nm. The results of the antioxidant study revealed that the plant extract possess effective antioxidant activity. HPTLC fingerprints were developed for rapid identification, authentication and control of adulteration of this crude drug, which is used as one of the ingredient in several ayurvedic formulations.

Desmostachya bipinnata, HPTLC fingerprinting, Antioxidant activity, DPPH.

"Antioxidant and HPTLC fingerprinting analysis of Desmostachya bipinnata", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.6, Issue 6, page no.127-130, June 2019, Available :http://www.jetir.org/papers/JETIR1907N57.pdf

"Antioxidant and HPTLC fingerprinting analysis of Desmostachya bipinnata", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.6, Issue 6, page no. pp127-130, June 2019, Available at : http://www.jetir.org/papers/JETIR1907N57.pdf