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June-2019
eISSN: 2349-5162
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Journal of Emerging Technologies and Innovative Research ( An International Scholarly Open Access Journal, Peer-reviewed, Refereed Journal )
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UGC Approved Journal no 63975(19)
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UGC and ISSN approved 7.95 impact factor UGC Approved Journal no 63975
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Published Paper ID:
JETIR1907R81
Registration ID:
224406
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Title
EVALUATION OF BIOACTIVE COMPOUNDS FROM NEWLY ISOLATED Haematococcus sp. BY IN-VITRO CELL CYTOTOXICITY ASSAY WITH FIBROBLAST AND NEUROBLASTOMA CELL LINES
Authors
Abstract
Present study deals with extraction and isolation of bioactive compounds from indigenous green microalgae Haematococcus sp. isolated from Karnataka, Dharwad. Study objectives mainly focused on investigate the efficacy of compound in crude form. Isolated strain cultivated in nutrient stress conditions in laboratory using mixotrophic cultivation. In stress condition isolate synthesized secondary carotenoids, and these carotenoids extracted using organic solvent . extract separated by TLC and used as crude drug to examine cytotoxic effects on fibroblast and cancer cell lines. Two cell lines NIH/3T3 and SKNSH used for in vitro cell viability study by MTT assay. Results indicated that the carotenoid rich crude drug have potential cytotoxic effect on SKNSH cell lines at concentration 800 and 1000µg/ml compared with cytotoxic effect of standard anticancer drug Cisplatin at concentration 15µg/ml. It found that the crude drug is non-cytotoxic to normal fibroblast at low concentration 200µg/ml cell lines but cytotoxic to neuroblastoma SKNH cell lines at low concentration 200-400 µg/ml. This property makes the crude drug to be used as potential bioactive anticancer natural supplement.
Key Words
Crude drug ,Carotenoids, Haematococcus sp., MTT, NIH/3T3 and SKNSH
Cite This Article
"EVALUATION OF BIOACTIVE COMPOUNDS FROM NEWLY ISOLATED Haematococcus sp. BY IN-VITRO CELL CYTOTOXICITY ASSAY WITH FIBROBLAST AND NEUROBLASTOMA CELL LINES", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.6, Issue 6, page no.503-506, June 2019, Available :http://www.jetir.org/papers/JETIR1907R81.pdf
ISSN
2349-5162 | Impact Factor 7.95 Calculate by Google Scholar
An International Scholarly Open Access Journal, Peer-Reviewed, Refereed Journal Impact Factor 7.95 Calculate by Google Scholar and Semantic Scholar | AI-Powered Research Tool, Multidisciplinary, Monthly, Multilanguage Journal Indexing in All Major Database & Metadata, Citation Generator
Cite This Article
"EVALUATION OF BIOACTIVE COMPOUNDS FROM NEWLY ISOLATED Haematococcus sp. BY IN-VITRO CELL CYTOTOXICITY ASSAY WITH FIBROBLAST AND NEUROBLASTOMA CELL LINES", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.6, Issue 6, page no. pp503-506, June 2019, Available at : http://www.jetir.org/papers/JETIR1907R81.pdf
Publication Details
Published Paper ID: JETIR1907R81
Registration ID: 224406
Published In: Volume 6 | Issue 6 | Year June-2019
Page No: 503-506
Country: -, -, - .
Area: Engineering
ISSN Number: 2349-5162
Publisher: IJ Publication
Published Paper URL :: https://www.jetir.org/view?paper=JETIR1907R81
Published Paper PDF: https://www.jetir.org/papers/JETIR1907R81
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