• Leena Koshti
• Darshana Patil
• Avinash Patil

Gymnema sylvestre R. Br. (Family: Asclepiadaceae), is a rare medicinal plant with significant medicinal attributes due to its principle phytoconstituent- “Gymnemic acid”. The objective of the present study was to isolate and quantify Gymnemic Acid through Gymnemagenin in leaf and leaf calli of Gymnema sylvestre R. Br. as per ICH (International Conference on Harmonization) guidelines. The Extraction of Gymnemic acid involved simple solvent extraction, followed by successive basic and acidic hydrolysis process. A precise and rapid high-performance thin-layer chromatography (HPTLC) method was developed and validated for Gymnemic acid analysis. The method involved separation on TLC aluminium precoated silica gel 60 F254 plate using ethyl acetate and methanol [5:6 (v/v)] as mobile phase. The purity and identity of peaks of leaf and callus cultures were confirmed by matching Rf values and UV-spectrum with authentic standard Gymnemic acid. The method showed good linear relationship in the range 0.3-0.7 µg/band with r 2 0.99. LOD and LOQ for Gymnemic acid was recorded as 52.8 ng/band and 160 ng/band respectively. The proposed HPTLC method was found to be simple and accurate to use and could be successfully used for routine quality control analysis of herbal material as well as formulations containing Gymnemic acid.

Gymnema sylvestre R. Br.; Gymnemic acid; Gymnemagenin; Callus; high-performance thin-layer chromatography

"Development Of Validated Hptlc For Simultaneous Quantification Of Gymnemic Acid Through Gymnemagenin In Leaf And Leaf Callus Of Gymnema Sylvestre R. Br.", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.6, Issue 6, page no.155-165, June-2019, Available :http://www.jetir.org/papers/JETIR1907S92.pdf

"Development Of Validated Hptlc For Simultaneous Quantification Of Gymnemic Acid Through Gymnemagenin In Leaf And Leaf Callus Of Gymnema Sylvestre R. Br.", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.6, Issue 6, page no. pp155-165, June-2019, Available at : http://www.jetir.org/papers/JETIR1907S92.pdf