• MADIREDDY ALEKHYA

ABSTRACT A RP-HPLC procedure is developed, validated and applied for simultaneous estimation of acetaminophen and benzhydrocodone in tablets. Procedure is based on separation and analysis of acetaminophen and benzhydrocodone in C18 column and 0.1MK2HPO4: methanol (60:40 v/v) mixture as stationary and mobile phase, respectively. The elution time values for acetaminophen and benzhydrocodone were 3.692 min and 4.956 min, respectively. Linear ranges for acetaminophen and benzhydrocodone are 162.5-487.5 µg/ml and 3.06-9.18  µg/ml, respectively. The values of sensitivity were 0.311 µg/ml (LOD) and 1.035 µg/ml (LOQ) for acetaminophen and 0.036 (LOD) µg/ml and 0.121 µg/ml (LOQ) for benhydrocodone. Validation parameters are tested using guidelines of ICH. The validation values obtained are well acceptable. The method proved as suitable procedure for assay of acetaminophen and benzhydrocodone in tablet dosage forms with good assay percent values.

Key words: acetaminophen, benzhydrocodone, Tablets RP-HPLC, K2HPO4: methanol, Validation

"RP-HPLC METHOD DEVELOPMENT AND VALIDATION FOR SIMULTANEOUS ESTIMATION OF ACETAMINOPHEN AND BENZHYDROCODONE IN PHARMACEUTICAL FORMULATIONS", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.6, Issue 6, page no.627-635, June-2019, Available :http://www.jetir.org/papers/JETIR1907T63.pdf

"RP-HPLC METHOD DEVELOPMENT AND VALIDATION FOR SIMULTANEOUS ESTIMATION OF ACETAMINOPHEN AND BENZHYDROCODONE IN PHARMACEUTICAL FORMULATIONS", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.6, Issue 6, page no. pp627-635, June-2019, Available at : http://www.jetir.org/papers/JETIR1907T63.pdf