• Sanghapal S. Kshirasagar
• Vishwas S. Shembekar

For body’s energy requirement we need regular supply of Glucose. To transport glucose, human serum albumin (HSA) is important & most abundant plasma protein. During transportation non-enzymatic glycation of protein occurs, which is a multi-step process, and is the first event in case of hyperglycemia. Accordingly, it yields advanced glycation end-products (AGEs), which can severely alter protein structure. Different methods have been used for determination of glycation of proteins and related AGEs like, Enzyme-linked immunosorbent assay (ELISA), High-performance liquid chromatography (HPLC) and mass spectrometry. However, their instrumentation and high cost of analysis limited their routine usefulness in analytical laboratories. Therefore, alternative methods to study glycation for routine use are desirable. Thus, the purpose is to develop rapid, simple, and sensitive methods for the determination glycation of human serum albumin by using a sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) & spectrofluorometric technique. In our study we have observed that, the molecular weight of glycated HSA has increased due to the formation of advanced glycation end products, and hence non-glycated HSA can be compared with properly glycated HSA by using SDS- PAGE. Similarly, fluorescence intensity of glycated HSA has increased using wavelengths 420 nm to 600 nm and excitation at 470 nm.

Analysis, Human serum albumin, Glycation, Glucose, Fluorescence Spectroscopy, SDS-PAGE

"ANALYSIS OF GLYCATED HUMAN SERUM ALBUMIN BY ELECTROPHORESIS AND FLUORESCENCE SPECTROSCOPY", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.6, Issue 6, page no.727-732, June-2019, Available :http://www.jetir.org/papers/JETIR1907T76.pdf

"ANALYSIS OF GLYCATED HUMAN SERUM ALBUMIN BY ELECTROPHORESIS AND FLUORESCENCE SPECTROSCOPY", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.6, Issue 6, page no. pp727-732, June-2019, Available at : http://www.jetir.org/papers/JETIR1907T76.pdf