• KIRAN KACHHAP
• Dr. Pallavi Sharma
• Prof. (Dr.) Amarendra Narayan Misra

Our experiment was focused on the enhancement of callus growth in combination of different concentration of auxin (2,4-D) and cytokinin (Kinetin) in invitro culture of Ocimum sanctum leaf explants. Various concentration of combination of 2,4-D (0.2,0.4 µg/ml) and Kinetin (0.1-0.5 µg/ml) are used in Murashige and skoog (MS) media. We find callus was obtained from O. sanctum leaf explants in all media supplemented with combination of 2,4-D and Kin were friable and whitish green in colour and maximum callus was obtained in the combination of 0.4 mg/ml 2,4-D and 0.2 μg/ml Kin concentration. Key words: Ocimum sanctum, 2,4-D, Kinetin, Callus

Ocimum Sanctum, Callus, 2,4-D, Kinetin

"Influence of different combinations of 2,4-D and Kin on callus induction, texture and colour of callus in Ocimum sanctum leaf explants.", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.6, Issue 6, page no.43-47, June 2019, Available :http://www.jetir.org/papers/JETIR1907U45.pdf

"Influence of different combinations of 2,4-D and Kin on callus induction, texture and colour of callus in Ocimum sanctum leaf explants.", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.6, Issue 6, page no. pp43-47, June 2019, Available at : http://www.jetir.org/papers/JETIR1907U45.pdf