• TEJA KUMAR REDDY KONATHAM
• jyoshna

guanpheinsin Guaifenesin can be analyzed by UV detection, HPTLC, HPLC individually and combination with other drugs in bulk material and pharmaceutical forms. A comprehensive, validated and simple analytical method development and validation of Guaifenesin is, therefore, crucial. No economic simple and precise HPLC method was there for evaluation of Guaifenesin in bulk and pharmaceutical dosage forms. Therefore, in proposed project a successful attempt has been made to develop, simple, accurate, and economic method for analysis of Guaifenesin tablets.The High performance liquid chromatography equipped with UV detector and with Chromatographic Workstation Software. The chromatograms of Guaifenesin were found to be satisfactory with Cosmosil Packed Column 5C18 – MS - II (4.6 x 250mm) the resolution of the proposed method was found to be satisfactory with peak showing complete base line separation. The proposed system of mobile phase and stationary phase was suitable for this estimation. System suitability parameters were within the limits as indicated by good number of theoretical plates. The accuracy of the method was determined by recovery with spiked concentrations at 50%, 100% and 150% levels. The recovery of drug was well within the acceptance limits of 98%-102%. The method is robust as observed from insignificant variation in the results of analysis on changes in flow rate, temperature, mobile phase composition and pH of the buffer. The linearity was observed over a wide concentration range of 10 - 35µg/ml.

guanphensin,accuracy,precision robustness,flowrate ,acetone ,sodium hydroxide ,methanol hplc grade water

"Development and validation of gaunpheinsin by using hplc", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.6, Issue 6, page no.144-183, June 2019, Available :http://www.jetir.org/papers/JETIR1907U57.pdf

"Development and validation of gaunpheinsin by using hplc", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.6, Issue 6, page no. pp144-183, June 2019, Available at : http://www.jetir.org/papers/JETIR1907U57.pdf