• Priya Prakash
• Deepalakshmi K

ABSTRACTS Carotenoids are the natural pigments present in many vegetables & fruits. They act as provitamin A and food colorants & are being extensively studied for their potential role in reducing the risk for cancer and other chronic diseases. The study was to estimate & purify the amount of carotenoids using solvent extraction followed by TLC. A comparison of the estimated amounts of carotenes with the other samples was made. The solvent extraction was done using hexane: acetone (1:1) along with ethanol, 10% NaCl solution. Silica gel & calcium sulphate hemihydrates were used in the ratio 4:1 along with double the amount of water to prepare the thin layer chromatography (TLC) plates. The mobile phase consisted of a mixture of hexane and acetone in the ratio of 3:2. The experimental yielded appreciable results and the samples were graded in the order of their respective carotene content.

Key words: - Carotenoids, Chlorophyll, TLC etc.

"“Extraction, Purification and Comparison of Carotenoids from Some Vegetables, Fresh Leafy Vegetables and Fruits”", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.6, Issue 6, page no.305-317, June 2019, Available :http://www.jetir.org/papers/JETIR1907U73.pdf

"“Extraction, Purification and Comparison of Carotenoids from Some Vegetables, Fresh Leafy Vegetables and Fruits”", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.6, Issue 6, page no. pp305-317, June 2019, Available at : http://www.jetir.org/papers/JETIR1907U73.pdf