• Dr. DEBASREE GHOSH
• MUKTA GUHA

Milk samples (raw cow and buffalo milk, processed milk) were collected from different areas of Shyamnagar city, North 24 Parganas, India. Adulterations (urea, cane sugar, starch, glucose, formalin, and coloring agent) of different milk samples were analysed. Microbial count was measured before and after the addition of Mentha arvensis (pudina) and Ocimum sanctum (tulsi) extract. The investigation was undertaken to explore the possibilities of utilizing Pudina and tulsi extracts in different type of milk samples to improve the shelf life of milk and improve the health benefits. Mentha arvensis (Pudina) and Ocimum sanctum (Tulsi) leaves extract were prepared through the mortar pestles. The extracts were mixed in milk samples by taking its proportion 2g, 3g and 5g in 10 ml milk samples. The Lactobacillus or NCDC-167 (BD4) culture was propagated in 90 ml sterile de Man-Rogosa Sharpe (MRS) broth and Yeast and mold cultures were propagated in 90 ml sterile Dextrose broth. Enumeration of lactobacillus, yeast and mould count of milk were determined by using pour plate method by using the Man-Rogosa Sharpe (MRS) agar for Lactobacillus and Dextrose agar for yeast and mould count. From the observation it was concluded that after adding the Pudina and Tulsi extract the Lactobacillus and yeast and mould count were decreased, with increase the level of both extractions the microbial counts were decreased largely. Pudina and tulsi may have the similar function to decrease the bacterial count due to their antimicrobial properties.

Mentha extract, Ocimum extract, cow milk, Lactobacillus, yeast.

"Studies on Antimicrobial Effect of Mentha arvensis (pudina) and Ocimum sanctum (tulsi) in Cow Milk", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.6, Issue 6, page no.215-224, June 2019, Available :http://www.jetir.org/papers/JETIR1907V34.pdf

"Studies on Antimicrobial Effect of Mentha arvensis (pudina) and Ocimum sanctum (tulsi) in Cow Milk", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.6, Issue 6, page no. pp215-224, June 2019, Available at : http://www.jetir.org/papers/JETIR1907V34.pdf