• Naziya Nishat Mohammad Aziz Rehman
• P.P.Dixit

Microbial secondary metabolites have been known for their miraculous powers and have been exploited in almost all fields of human welfare. Many have a firm grip in pharmaceutical and other industries. In today’s scenario, alpha glucosidase inhibitors have drawn ample scope as they have considered good candidates to treat diabetes. Understanding the importance of alpha glucosidase inhibitor and keeping in mind potentials of microbial secondary products, this research study was undertaken. The aim of this research study was to isolate pigment producing microbes and to screen out the pigments that have alpha glucosidase inhibitory activity. One bacterium that was able to produce dark maroon color pigment was isolated. A protocol was developed to completely extract the pigment. The pigment was purified by silica gel column chromatography and purity of each fraction was confirmed by performing ascending thin layer chromatography. Purified fractions obtained were tested for alpha glucosidase inhibitory activity. The pigment was found to possess good alpha glucosidase inhibitory activity. As pigment had a good activity, attempts were made to enhance the pigment production in less time. It was observed in the research process that one of the sugar alcohols has positive effect on pigment production so considering this fact, we speculated that other sugar alcohols might increase the pigment yield substantially. To check and test out speculation, we performed an experiment and our postulation turned into a fact. In presence of dulcitol the pigment production was doubled. The pigment was produced at large scale within short time by using dulcitol in the medium.

Bacteria ,pigment, glucosidase inhibitory activity

"Enhanced Production of bacterial pigment possessing alpha glucosidase inhibitory activity", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.6, Issue 6, page no.1011-1024, June 2019, Available :http://www.jetir.org/papers/JETIR1908303.pdf

"Enhanced Production of bacterial pigment possessing alpha glucosidase inhibitory activity", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.6, Issue 6, page no. pp1011-1024, June 2019, Available at : http://www.jetir.org/papers/JETIR1908303.pdf