• Anupriya B.Chudiwal
• Dr.Smita M.Dharamadhikari

The exopolysaccharides (EPS) producing ability was evaluated by nutritional and chemically defined media by the laboratory isolated haloalkalotolernt Psudomonas aeroginosa which gave highest yield EPS . It was identified as Psudomonas aeroginosa on selective media and 16S rDNA gene sequencing. The favorable conditions for EPS yield by the microorganism growth in a chemically defined medium were tested with carbon, nitrogen, sodium chloride, metal ions which is a significant factors for EPS production . Glucose was the most favorable carbon source for EPS production and maximum production (4.6 g/L) was recorded with 1.5% glucose. Among different nitrogen sources, Ammonium sulfate at 0.7% level was proved to be the best for EPS production which enhanced the EPS production to3.98 g/L, while the metal ions (MgSo4 )were also found to be favorable to growth and EPS production. An EPS production of 4.70 g/L was achieved when the growth medium was supplemented with 3.5% NaCl. The maximum EPS production was viewed at the 72 hrs of incubation at submerged condition

Psudomonas aeroginosa exopolysaccharides, optimization

"Optimization Of Nutritional Conditions For Exopolysaccharide Production By Using Marine Psudomonas Aeroginosa Isolated From Anjerle Beach", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.6, Issue 6, page no.186-190, June 2019, Available :http://www.jetir.org/papers/JETIR1908491.pdf

"Optimization Of Nutritional Conditions For Exopolysaccharide Production By Using Marine Psudomonas Aeroginosa Isolated From Anjerle Beach", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.6, Issue 6, page no. pp186-190, June 2019, Available at : http://www.jetir.org/papers/JETIR1908491.pdf