• Menaga M
• Leon Stephan Raj T
• Jebarubi E

The genus Tephrosia is one of the important genera of the family fabaceae and having 400 species throughout the world. Most of the species of the genera are morphologically alike but differ only in few characters. In the present investigation, we have analyzed the genetic variability of selected five species (T. purpurea, T. villosa, T. spinosa, T. maxima and T. procumbens) using ISSR (Inter Simple Sequence Repeat) markers. The species were collected from in and around of Tirunelveli districts of Tamil Nadu. There are ten ISSR primers were screened for the study out of which five reproducible primers were used for further analysis. The five primers were produced 29 scorable bands with five Tephrosia species. The number of polymorphic loci was 13 and polymorphic percentage was 56.52%. The genetic distance between the Tephrosia species was ranged from 0.4229 to 0.8023 and genetic diversity was ranged from 0.4483 to 0.6552. The mean value of overall observed and effective number of alleles was about 1.9310 and 1.6410. Nei’s overall genetic diversity and Shannon information index was 0.3697 and 0.5433. The similarity matrix obtained in the present study was used to construct a dendrogram with the UPGMA method and resulted in their distant clustering in the dendrogram. The results indicated that the similarity and dissimilarity between selected Tephrosia species.

Genetic diversity, Molecular marker, ISSR, Tephrosia, Tirunelveli

"ASSESSMENT OF GENETIC DIVERSITY OF SELECTED TEPHROSIA SPECIES FROM TIRUNELVELI DISTRICT OF TAMIL NADU", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.6, Issue 6, page no.913-917, June-2019, Available :http://www.jetir.org/papers/JETIR1908737.pdf

"ASSESSMENT OF GENETIC DIVERSITY OF SELECTED TEPHROSIA SPECIES FROM TIRUNELVELI DISTRICT OF TAMIL NADU", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.6, Issue 6, page no. pp913-917, June-2019, Available at : http://www.jetir.org/papers/JETIR1908737.pdf