• Rashmi A Wanjari
• Arti S. Shanware
• Sanjay J. Dhoble

Culturable luminous bacterial strains serves as efficient biosensor in different monitoring systems specially radiation pollution. The aim of this study was to carry out an effective method for the isolation and characterization of novel luminescent bacterial species from sea water sample Tarkarli beach of Sindhudurg, India and efforts have been made to isolate luminescent bacterial species by using simplest possible medium and comparative assessment of the propensity to emit light under altering culture conditions such as temperature, medium composition, pH etc. The preliminary screening and molecular characterization was done following the standard techniques. Further, 16s rRNA amplified genes were sequenced and then analyzed by BLAST for similarity search followed by Phylogenetic analysis using MEGA software revealed that the strain is Vibrio natriegens which was successfully isolated from Tarkarli beach of Sindhudurg, India. By utilizing outcomes of luminescence intensity measurements of isolated bacteria acquired with luminometer, the strains and media providing stern and steady luminescence were endeavored in this study.

Bioluminescent bacteria; Vibrio natriegens; 16S rRNA gene analysis; Biosensor.

"Effects of some physicochemical parameters and UV irradiation on Bioluminescence", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.6, Issue 6, page no.365-376, June 2019, Available :http://www.jetir.org/papers/JETIR1908800.pdf

"Effects of some physicochemical parameters and UV irradiation on Bioluminescence", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.6, Issue 6, page no. pp365-376, June 2019, Available at : http://www.jetir.org/papers/JETIR1908800.pdf