• Ms.Tanvi Kishor Sawant
• Dr. Shruti Laxman Samant

PHA(Polyhydroxyalkanoate) bioplastic holds the potential to replace the petroleum plastic by virtue of its high elasticity, stress resistance and low brittleness. However, for its large-scale production, cost of production is a major bottleneck of which the carbon substrate alone accounts for 28-50% of the production cost. So, studies are carried out to test the PHA bioplastic production by microbial culture on crude carbon substrates such as whey, starch, oil, lignocellulosic material and sugar wastes. In this study, one such attempt was made to produce PHA bioplastic from Pseudomonas putida ATCC 49128 grown on waste carbon substrate such as fruit and vegetable peel. The effect of nutrient deficiency and excess on PHA yield was also studied, wherein it was found that on imparting nutrient deficiency to grown culture medium PHA yield increased and presence of easily utilizable nitrogen source like peptone inhibited the PHA production. By GC-MS analysis, the PHA produced was found to be mcl-PHA (medium chain length-PHA) consisting of Hexadecanoate C-16 monomer and Octadecanoate C-18 monomer constituent which directly corresponded to the waste fruit and vegetable peel substrate fatty acid content. PHA bioplastic exhibit wide range of application also its newly recognized potential to be recycled into novel biofuels is entering a new development phase.

Polyhydroxyalkanoate, bioplastic, crude waste substrate, cost reduction

"Cost Reduction and study of PHA bioplastic production from Pseudomonas putida ATCC 49128 utilizing fruit and vegetable peel waste as carbon substrate", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.6, Issue 6, page no.976-982, June-2019, Available :http://www.jetir.org/papers/JETIR1908936.pdf

"Cost Reduction and study of PHA bioplastic production from Pseudomonas putida ATCC 49128 utilizing fruit and vegetable peel waste as carbon substrate", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.6, Issue 6, page no. pp976-982, June-2019, Available at : http://www.jetir.org/papers/JETIR1908936.pdf