• Sahil Abbas
• Girendra Gautam
• Pawan Kumar Gautam

There is an increase or discovery of different diseases which causing great harm to society. The past researches indicated that huge number of antibiotics was produced by Gram +ve ike bacteria known as Actinomycetes. Among all more than 50% of the known antimicrobial compounds were produced by Actinomycetes only. These are a specific type of class of prokaryotes forming thread like structure at some stage of their growth, so refereed as filamentous prokaryotes. This class or group is an actively produce of different types of enzymes, enzyme inhibitors, growth promoter and antibiotics etc. In our study screening of Actinomycetes was performed by using different river sediments. Soil samples was collected from river Godaveri and Krishna and stored in the UV and alcohol sterilized Poly bags. Soil samples was serially diluted upto 10-6 and 1 ml from each dilution was placed on different isolation media like starch Casein agar, Albumin media and YMA media, consisting of antifungal agent Nystatin 50 µg/ ml, by pour plate technique. The plates were incubated at different temperature ranges 180C to 280C upto 7-14 days. There were 3 actinomycetes were isolated and these were streak on solidified Bennet agar media at straight line and plates were kept for incubation in incubator at 370C for nearly about 3 days. Identification of actinomycetes was performed using Gram’s staining.

Keywords: Actinomycetes, River sediments, Nutrient media, Identification.

"SCREENING AND ISOLATION OF ACTINOMYCETESS FROM RIVER SEDIMENTS", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.6, Issue 6, page no.695-707, June-2019, Available :http://www.jetir.org/papers/JETIR1908B90.pdf

"SCREENING AND ISOLATION OF ACTINOMYCETESS FROM RIVER SEDIMENTS", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.6, Issue 6, page no. pp695-707, June-2019, Available at : http://www.jetir.org/papers/JETIR1908B90.pdf