UGC Approved Journal no 63975

ISSN: 2349-5162 | ESTD Year : 2014
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Published in:

Volume 6 Issue 6
June-2019
eISSN: 2349-5162

UGC and ISSN approved 7.95 impact factor UGC Approved Journal no 63975

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Published Paper ID:
JETIR1908C65


Registration ID:
301873

Page Number

501-515

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Title

Efficient immobilization of Diamine Oxidase by entrapment and study of optimum parameters

Abstract

The Presence of Diamine oxidase (DAO) has gained tremendous attention in food and allied industries for guarding food freshness and safety.All the commercial application of enzymes as biocatalysts requires simple but efficient methods to immobilize the enzyme to yield highly stable and active biocatalysts. In this study, DAO was extracted, purified and immobilized by entrapment in matrix sodium alginate in presence of the enzyme. The optimal parameters of free and immobilized DAO activity were evaluated against the substrate, Putrescinedihydrochloride and the results showed that the stabilities were significantly enhanced compared with free counterpart. The optimum working pH was 7.5 where both free and immobilized enzyme showed their maximum activity.. Free enzyme showed maximum activity at 37°C while immobilized enzyme showed stable activity at a wide range of temperature. Residual activity of the immobilized enzyme was 59% of the initial activity after being recycled 10 times. Therefore, this method may provide an excellent support for enzyme immobilization having better catalytic ability in large scale applications in biotechnological industries.

Key Words

Diamine oxidase enzyme; entrapment immobilization, Comparative Study of Enzyme properties

Cite This Article

"Efficient immobilization of Diamine Oxidase by entrapment and study of optimum parameters", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.6, Issue 6, page no.501-515, June-2019, Available :http://www.jetir.org/papers/JETIR1908C65.pdf

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2349-5162 | Impact Factor 7.95 Calculate by Google Scholar

An International Scholarly Open Access Journal, Peer-Reviewed, Refereed Journal Impact Factor 7.95 Calculate by Google Scholar and Semantic Scholar | AI-Powered Research Tool, Multidisciplinary, Monthly, Multilanguage Journal Indexing in All Major Database & Metadata, Citation Generator

Cite This Article

"Efficient immobilization of Diamine Oxidase by entrapment and study of optimum parameters", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.6, Issue 6, page no. pp501-515, June-2019, Available at : http://www.jetir.org/papers/JETIR1908C65.pdf

Publication Details

Published Paper ID: JETIR1908C65
Registration ID: 301873
Published In: Volume 6 | Issue 6 | Year June-2019
DOI (Digital Object Identifier):
Page No: 501-515
Country: --, -, - .
Area: Engineering
ISSN Number: 2349-5162
Publisher: IJ Publication


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