UGC Approved Journal no 63975(19)

ISSN: 2349-5162 | ESTD Year : 2014
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Published in:

Volume 6 Issue 6
June-2019
eISSN: 2349-5162

UGC and ISSN approved 7.95 impact factor UGC Approved Journal no 63975

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Published Paper ID:
JETIR1908E14


Registration ID:
404324

Page Number

100-111

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Title

Characterization, Purification, and Production of Alkaline Amylase from Bacteria via Solid-State

Abstract

Amylase is used in a variety of industries, including detergents, food, fermentation, textile paper, and pharmaceuticals. Bacteria generating amylase enzyme was investigated in this study from the gut of fish (Channa punctatus). A shake flask fermentation was used to make the extracted amylase enzyme, and ammonium sulfate precipitation was used to purify it. The activity of the enzyme was determined using a synthetic carbohydrates starch substrate. The partially purified enzyme exhibits maximum activity at the optimum pH 7-11, temperature 28-37°C, and substrate concentration of one percent Starch, and the result also shows that maximum alkaline amylase synthesis occurs in solid-state conditions at pH 11, incubated for 5 days at room temperature. Under the solid-state fermentation process, bacteria produce the most amylase. The influence of activators and inhibitors on amylase enzyme production was also investigated, with activators MgCl2 and CaCl2 increasing enzyme activity and inhibitors SDS and EDTA decreasing enzyme activity.

Key Words

Alkaline amylase, Bacteria, solid-state fermentation, enzyme activity

Cite This Article

"Characterization, Purification, and Production of Alkaline Amylase from Bacteria via Solid-State", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.6, Issue 6, page no.100-111, June-2019, Available :http://www.jetir.org/papers/JETIR1908E14.pdf

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2349-5162 | Impact Factor 7.95 Calculate by Google Scholar

An International Scholarly Open Access Journal, Peer-Reviewed, Refereed Journal Impact Factor 7.95 Calculate by Google Scholar and Semantic Scholar | AI-Powered Research Tool, Multidisciplinary, Monthly, Multilanguage Journal Indexing in All Major Database & Metadata, Citation Generator

Cite This Article

"Characterization, Purification, and Production of Alkaline Amylase from Bacteria via Solid-State", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.6, Issue 6, page no. pp100-111, June-2019, Available at : http://www.jetir.org/papers/JETIR1908E14.pdf

Publication Details

Published Paper ID: JETIR1908E14
Registration ID: 404324
Published In: Volume 6 | Issue 6 | Year June-2019
DOI (Digital Object Identifier):
Page No: 100-111
Country: -, -, India .
Area: Engineering
ISSN Number: 2349-5162
Publisher: IJ Publication


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