• DR ASHWANI SHARMA
• PROF JAMES GOMES

Abstract: In this study the production of lovastatin by submerged fermentation using Aspergillus sp. wascarried out in shake flask. The objective of this study was to improve lovastatin production by screening and optimization of carbon and nitrogen sources. Carbon sources such as glucose, lactose, starch, and molasses alone and in combination and different nitrogen sources (yeast extract, soybean meal, ammonium nitrate, corn steep liquor, sodium glutamate) were evaluated in terms of lovastatin production. Other medium constituents and culture conditions were held constant while only carbon and nitrogen sources were changed in separate experiments. The shake flask study was carried out for a period of 168 h. The highest lovastatin production of 541 mg/l was obtained at 144 h, using the medium containing glucose and lactose as carbon source and Na-glutamate as a nitrogen source. A response surface analysis was performed based on the central composite design to determine the C: N ratio optimal for lovastatin production. A cubic model was developed to elucidate the combined effect of glucose, lactose and sodium glutamate concentrations. The optimized value obtained by the statistical analysis showed that glucose at 22.78 gm/l, lactose 26 gm/l and 14.48 gm/l Na-glutamate was optimum for the maximum lovastatin production after 144 h of fermentation.

Lovastatin; Carbon and Nitrogen Sources; Statistical design; Fermentation; Aspergillus sp.

"Screening and Optimization of Carbon and Nitrogen Source for IncreasingLovastatin Production by Submerge Fermentation Using Aspergillus Sp. ", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.7, Issue 2, page no.152-156, February-2020, Available :http://www.jetir.org/papers/JETIR2002024.pdf

"Screening and Optimization of Carbon and Nitrogen Source for IncreasingLovastatin Production by Submerge Fermentation Using Aspergillus Sp. ", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.7, Issue 2, page no. pp152-156, February-2020, Available at : http://www.jetir.org/papers/JETIR2002024.pdf