• Vitthal Sahebrao Gunjal
• Dr. Vipulkumar Prahladbhai Patel
• Avani Rajendraprasad Vaishnav
• Santoshi Madhukarrao Deshmukh

A Simple, accurate, and precise reversed phase high performance liquid chromatographic method was develope for simultaneous determination of Halobetasol Propionate and preservative (Methyl Paraben and Propyl Paraben) in formulation. In RP- HPLC the separation was carried out using mobile phase consisting of Acetonitrile: Methanol: Buffer (60:15:25v/v/v) and the Buffer was 0.08 %( v/v) Triflouroacetic acid. The column used was Inertsil ODS 3V, C18, 250 X 4.6mm, 5μ with flow rate 1ml/min. using UV detector at 240 nm. The retention time of Methyl paraben, Propyl paraben and Halobetasol Propionate were found to be around 3.43min, 4.45min and 7.06min respectively. For Linearity, R2 value was found to be 0.9971 for Methyl Paraben, 0.9986 for Propyl Paraben and 0.9992 for Halobetasol Propionate. % Assay was found to be 102.00%, 102.91% and 100.94% for Methyl Paraben, Propyl Paraben and Halobetasol Propionate respectively. Percentage recovery for Methyl Paraben was 98.52– 100.84%, for Propyl Paraben, it was found to be 98.40– 100.06% and for Halobetasol Propionate it was 99.04- 101.45%. The results of the study showed that the proposed RP-HPLC method was found to be simple, sensitive, precise and accurate and also useful for the routine analysis of Halobetasol Propionate and preservative (Methyl Paraben and Propyl Paraben) in dosage form.

Halobetasol Propionate, Methyl Paraben, Propyl Paraben, RP-HPLC, Preservative.

"Development and validation of RP HPLC method for determination of Halobetasol Propionate and preservative (Methyl Paraben and Propyl Paraben) in formulation.", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.7, Issue 4, page no.1113-1124, April-2020, Available :http://www.jetir.org/papers/JETIR2004348.pdf

"Development and validation of RP HPLC method for determination of Halobetasol Propionate and preservative (Methyl Paraben and Propyl Paraben) in formulation.", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.7, Issue 4, page no. pp1113-1124, April-2020, Available at : http://www.jetir.org/papers/JETIR2004348.pdf