• Sneha khadse
• Priyanka Titirmare

The present study deals with the isolation, production, purification and optimization of antibiotics from soil. The soil samples were collected from various places. Actinomycetes strains were isolated in specific medium using starch casein agar medium. The actinomycetes were screened with regard to potential against gram positive and gram negative bacteria. The purified actinomycetes strains were performed in biochemical tests. Gram staining, sugars fermentation, HS production, urease production, MR-VP test, indole production, citrate 2 utilization. Fermentation was carried out with starch casein broth and identified strain of actinomyces as inoculums. Antimicrobial activity of antibiotics was detected on Mueller Hinton Agar Media against E.coli. The purification of antibiotics was done with the help of activated charcoal. During the experiment a modified fermentation medium was formulated and activity of antibiotic produced in this medium was detected against E.coli. The isolated bacteria such as actinomycetesobtained from the soil.

“ISOLATION OF ANTIBIOTIC PRODUCING ACTINOMYCETES FROM SOIL”

"“ISOLATION OF “ISOLATION OF ANTIBIOTIC PRODUCING ACTINOMYCETES FROM SOIL”", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.7, Issue 5, page no.481-487, May-2020, Available :http://www.jetir.org/papers/JETIR2005073.pdf

"“ISOLATION OF “ISOLATION OF ANTIBIOTIC PRODUCING ACTINOMYCETES FROM SOIL”", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.7, Issue 5, page no. pp481-487, May-2020, Available at : http://www.jetir.org/papers/JETIR2005073.pdf