• SUNIL KUMAR
• RUCHI BANSAL

Abstract Background: Liver is a vital organ of the human body, and hepatotoxicity is major problem of liver and the current scenario is effected in this problem in a big ratio it has many causes, in this study the hepatotoxicity induced in rat to use CCl4 and its treated by hydro-alcoholic extract of C.tinctoria to given low and high dose and was to be evaluated by the comparison between disease and treatment group. Objective: The present study was design to carry out for the evaluation of hepatoprotective effects of hydro alcoholic leaves extract of Chrozophora tinctoria over the carbon tetrachloride (CCl4) induced liver damage in albino Wister rats. Methods: All the experimental Wister rats were randomly divided into five groups having six rats in each. Group I served as the normal control (Saline 0.9%) rat, Group II, Group III, Group IV and Group V was administered with CCl4which was prepared in 20% olive oil at a signal dose of 1.5ml/kg body weight by i.p. route for induction of hepatotoxicity. In an experimental protocol Group II serves as diseased control. Group III: was administered with hydro alcoholic extract of C.tinctoria (50mg/kg/day), Group IV: was treated with hydro alcoholic extract of C.tinctoria (100mg/kg/day). Group V: was treated with sylimarin (25mg/kg /day). Over the experimental period of 28 days blood sample were collected for biochemical analysis, and liver tissue were collected for biopsy. Result: After administration of CCl4 rats developed hepatotoxicity which evident by elevation in AST, ALT, ALP, total bilirubin, total proteins and the antioxidant enzyme parameters CAT, SOD, GGT, GST was decreases. Although administer of CCl4. Administration of hydro alcoholic extract of C.tinctoria (50mg/kg /day) shows improved in AST, ALT, ALP, total bilirubin, total proteins towards normal level and significantly increases (**p<0.05) the antioxidant enzyme parameters CAT, SOD, GGT, and GST. Similarly, administration of hydro alcoholic extract of C.tinctoria(100mg/kg/day) shows more significantly decrease (**p<0.05) in AST, ALT, ALP, total bilirubin, total protein towards normal level increases and more significantly (**p<0.05)the antioxidant enzyme parameters CAT, SOD, GGT, and GST in compare to standard as well as control groups. However, the albino Wister rats treated with high dose hydro alcoholic extract (C.tinctoria 100mg) showed more significant improvements as hepatoprotective than low dose of C.tinctoria i.e.50mg. Discussion: The hepatoprotective activity of hydro alcoholic extract of C.tinctoria 100mg could palliate the liver injuries perhaps by its phytoconstituents and antioxidant enzyme, hence it eliminating the deleterious effect of toxic metabolites from the CCl4.

Keywords: Chrozophora Tinctoria, Carbon Tetra Chloride, Hepatoprotective,AST, ALT, ALP.

"Pharmacological Potential of Chrozophora Tinctoria Extracts for the Management of Carbon Tetra Chloride (Ccl4) Induced Hepatotoxicity in Rat’s Model", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.7, Issue 6, page no.639-651, June-2020, Available :http://www.jetir.org/papers/JETIR2006087.pdf

"Pharmacological Potential of Chrozophora Tinctoria Extracts for the Management of Carbon Tetra Chloride (Ccl4) Induced Hepatotoxicity in Rat’s Model", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.7, Issue 6, page no. pp639-651, June-2020, Available at : http://www.jetir.org/papers/JETIR2006087.pdf