• Kapadia Komal K
• Khatri Riddhima P

The aim of this research was production and analysis of melanin pigment producing isolate from farm soil sample and study of its photo-protective role. A pigment producing isolate was screened on Tyrosine casein media from the soil samples collected from various farms. The bacteria were identified as Pseudomonas aeruginosa by molecular characterization. Production of pigment was carried out by Submerged fermentation (SF) technique. Optimization parameters like Carbon and Nitrogen sources, along with effect of incubation time on pigment production was also studied. Maximum production was observed at 192nd hour to the incubation and pigment production was observed in Starch and L-tyrosine containing medium. The pigment extracted was found to be water soluble and was confirmed to be photo-protective using UV-Vis spectroscopic studies. The pigment showed reducing power capacity (Oyaizu 1986) which serves as potent antioxidants. Fourier Transform Infra-red spectrum (FTIR) analysis confirmed the crude melanin extract obtained as melanin.

Melanin, L-tyrosine, Photo-protective, Antioxidants

"Production and analysis of melanin pigment from bacteria isolated from farm soil", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.7, Issue 6, page no.1382-1394, June-2020, Available :http://www.jetir.org/papers/JETIR2006203.pdf

"Production and analysis of melanin pigment from bacteria isolated from farm soil", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.7, Issue 6, page no. pp1382-1394, June-2020, Available at : http://www.jetir.org/papers/JETIR2006203.pdf