UGC Approved Journal no 63975

ISSN: 2349-5162 | ESTD Year : 2014
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Published in:

Volume 6 Issue 6
June-2019
eISSN: 2349-5162

UGC and ISSN approved 7.95 impact factor UGC Approved Journal no 63975

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Published Paper ID:
JETIR2006224


Registration ID:
234245

Page Number

1519-1530

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Title

Isolation and characterization of Rhizobium species from cultivated and wild leguminous plants

Abstract

Plant growth and development requires nitrogen as essential elements, which supplied by symbiotic bacteria rhizobia in association with leguminous plants. Plant growth promoting Rhizobacteria are capable of promoting plant growth by colonizing the plants root and enhances symbiotic and associative nitrogen fixation. This work aimed isolation, characterization and PGPR activity of the bacteria from cultivated and non-cultivated leguminous plants. Seven strains of Rhizobium species were isolated from six non-cultivated wild leguminous plants and three Rhizobium strains were isolated from cultivated leguminous plants. Plant root´s nodule collected from different regions of Ranchi, Jharkhand. Bacteria were isolated for its characterization and PGPR activities, such as IAA production, Phosphate solublization, HCN production, Ammonia production. During this work six non-cultivated leguminous plants were identified namely Tephrosia (Tephrosia purpurea), Tick clover (Desmodium tortuosum), Karanj (Pongamia pinnata), Shisham (Dalbergia sissoo), Lupin (Lupinus albus), Gulmohar (Delonix regia). From these plants seven Rhizobium species were isolated (RC1, RC2, RC3, RC4, RC5, RC6, RC7) and characterized. Three isolates (RC8, RC9, RC10) were also characterized from cultivated leguminous plants namely Dolichos bean (Lablab purpureus), Arhar (Cajanus arietinum), Gram (Cicer arietinum). These ten isolates showed PGPR activities, which confirmed through IAA, HCN and ammonia production tests. The aim of this study is to isolate Rhizobacteria from wild and cultivated leguminous plants, which have PGPR activity. Inoculum of all these Rhizobium species isolated from non-cultivated wild leguminous plants can exploited in plant growth promotion.

Key Words

Indole acetic acid, leguminous, non-cultivated, PGPRs, Rhizobium species, wild strain

Cite This Article

"Isolation and characterization of Rhizobium species from cultivated and wild leguminous plants ", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.6, Issue 6, page no.1519-1530, June-2019, Available :http://www.jetir.org/papers/JETIR2006224.pdf

ISSN


2349-5162 | Impact Factor 7.95 Calculate by Google Scholar

An International Scholarly Open Access Journal, Peer-Reviewed, Refereed Journal Impact Factor 7.95 Calculate by Google Scholar and Semantic Scholar | AI-Powered Research Tool, Multidisciplinary, Monthly, Multilanguage Journal Indexing in All Major Database & Metadata, Citation Generator

Cite This Article

"Isolation and characterization of Rhizobium species from cultivated and wild leguminous plants ", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.6, Issue 6, page no. pp1519-1530, June-2019, Available at : http://www.jetir.org/papers/JETIR2006224.pdf

Publication Details

Published Paper ID: JETIR2006224
Registration ID: 234245
Published In: Volume 6 | Issue 6 | Year June-2019
DOI (Digital Object Identifier):
Page No: 1519-1530
Country: Ranchi, Jharkhand, India .
Area: Biological Science
ISSN Number: 2349-5162


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