• Vidya N. Alhat
• Salim G. Patel
• Dushyant D. Gaikwad
• Suresh L. Jadhav

ABSTRACT The current work is carried out for determination and validation of zero-order derivative spectroscopy of Formoterol Fumarate Dihydrate (FFD) in bulk and formulation by using UV-Spectrophotometer. For this purpose, the wavelength of 214 nm was selected. Sodium phosphate buffer was used as a solvent throughout the work. Linearity was observed in the concentration range of 2-12 µg/ml for the method. FFD obeyed Lambert Beer’s law in the concentration range of 2 to 12 μg/mL (r² = 0.9993). The percent concentration in the Bulk drug was found to be 100.21 ± 0.03%, the percent concentration in marketed tablet Formulation was found 99.72 ± 0.02%. Parameters such as linearity, precision, accuracy, specificity, and ruggedness were studied as reported in the International Conference on Harmonization guidelines. The relative standard deviations for three replicate measurements in six concentrations of samples were found less than 2 %. The validation procedure confirms that this can be an appropriate method for their quantification in the formulation. The present method was found to be simple and linear which can be used for routine quality control analysis for spectrophotometric determination of zero-order derivative method in bulk or pharmaceutical formulation.

Keywords: Formetrol Fumarate Dihydrate (FFD), Antiasthmatic, Zero-Order derivative, λ max, UV.

"DEVELOPMENT AND VALIDATION OF ZERO ORDER UV SPECTROPHOTOMETRIC METHOD FOR ESTIMATION OF FORMOTEROL FUMARATE DIHYDRATE IN BULK AND IN PHARMACEUTICAL FORMULATION.", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.7, Issue 6, page no.1986-1994, June-2020, Available :http://www.jetir.org/papers/JETIR2006287.pdf

"DEVELOPMENT AND VALIDATION OF ZERO ORDER UV SPECTROPHOTOMETRIC METHOD FOR ESTIMATION OF FORMOTEROL FUMARATE DIHYDRATE IN BULK AND IN PHARMACEUTICAL FORMULATION.", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.7, Issue 6, page no. pp1986-1994, June-2020, Available at : http://www.jetir.org/papers/JETIR2006287.pdf