• Parul Bisht
• Archana Gahtori

Advantages such as simple sample preparation steps with no time-consuming extraction and separation lead to the more frequent use of simultaneous estimation procedure in case of the multicomponent pharmaceutical dosage forms which are accurate and precise. In the present study, a UV Spectrophotometric method has been developed and validated for the simultaneous estimation of teneligliptin hydrobromide and metformin hydrochloride in bulk and tablet dosage form. The estimation was performed by using two methods namely; Simultaneous equation method and Absorbance ratio method. Both the spectrophotometric methods employed preparation of dilutions using distilled water. In simultaneous equation method, absorbance was measured at 230nm and 245nm for both the drugs whereas, in absorbance ratio method the absorbance was measured at 230nm and 240nm for both the drugs. The calibration plot was found to be linear between concentration range 2-12 μg/ml for metformin and 5 -55μg/ml for teneligliptin with R2 = 0.999 for both the drugs. The amount of drugs estimated by both the methods was in good agreement with the label claim. The method was validated according to the ICH guidelines. Therefore, both the developed methods can be used for routine quality control analysis of Teneligliptin and metformin in bulk and pharmaceutical formulation.

Keywords: Teneligliptin HBr, Metformin HCl, Simultaneous equation, Absorbance ratio

"DEVELOPMENT AND VALIDATION OF UV-VISIBLE SPECTROPHOTOMETRIC METHOD FOR SIMULTANEOUS DETERMINATION OF TENELIGLIPTIN HYDROBROMIDE AND METFORMIN HYDROCHLORIDE IN ITS BULK AND DOSAGE FORM", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.7, Issue 6, page no.988-995, June-2020, Available :http://www.jetir.org/papers/JETIR2006475.pdf

"DEVELOPMENT AND VALIDATION OF UV-VISIBLE SPECTROPHOTOMETRIC METHOD FOR SIMULTANEOUS DETERMINATION OF TENELIGLIPTIN HYDROBROMIDE AND METFORMIN HYDROCHLORIDE IN ITS BULK AND DOSAGE FORM", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.7, Issue 6, page no. pp988-995, June-2020, Available at : http://www.jetir.org/papers/JETIR2006475.pdf