UGC Approved Journal no 63975(19)

ISSN: 2349-5162 | ESTD Year : 2014
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Published in:

Volume 7 Issue 6
June-2020
eISSN: 2349-5162

UGC and ISSN approved 7.95 impact factor UGC Approved Journal no 63975

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Published Paper ID:
JETIR2006554


Registration ID:
234763

Page Number

1521-1539

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Title

Development of a dominant SCAR marker associated with an allele of SV1 gene for sterility mosaic disease resistance in pigeonpea [Cajanus cajan (L.) Millsp]

Abstract

Pigeonpea sterility mosaic disease (PSMD) caused by pigeonpea sterility mosaic virus (PPSMV) is one of the major biotic concerns that leads to serious yield losses and, hence, poses a big challenge for pigeonpea production in the Indian subcontinent. In this study the inheritance, identification and development of molecular markers associated with Bidar isolate of PPSMV resistance gene by using F2 population was attempted. We reveal that PSMD is controlled by two genes, named as SV1 (inhibitory) and SV2 (resistant). Of the 300 RAPD primers, 32 primers recorded polymorphism in the parents, were screened using bulked segregant analysis method. Two markers (IABTPPN18827 and IABTPPAK191295) amplified susceptible parent specific amplicon and were associated with the PSMD responsive gene at a distance of about 12.5 cM and 10 cM respectively. We developed SCAR marker associated with the SV1 gene. Based on the sequencing data of the IABTPPN18827 marker, we successfully developed a dominant SCAR marker, FlntN18827, which was associated with SV1 gene at the distance of 12.5 cM. This SV1-SCAR marker will be a valuable tool for marker-assisted breeding in developing PSMD resistant pigeonpea.

Key Words

PIGEONPEA, PSMD, PPSMV, SV1, RAPD, SCAR

Cite This Article

"Development of a dominant SCAR marker associated with an allele of SV1 gene for sterility mosaic disease resistance in pigeonpea [Cajanus cajan (L.) Millsp] ", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.7, Issue 6, page no.1521-1539, June 2020, Available :http://www.jetir.org/papers/JETIR2006554.pdf

ISSN


2349-5162 | Impact Factor 7.95 Calculate by Google Scholar

An International Scholarly Open Access Journal, Peer-Reviewed, Refereed Journal Impact Factor 7.95 Calculate by Google Scholar and Semantic Scholar | AI-Powered Research Tool, Multidisciplinary, Monthly, Multilanguage Journal Indexing in All Major Database & Metadata, Citation Generator

Cite This Article

"Development of a dominant SCAR marker associated with an allele of SV1 gene for sterility mosaic disease resistance in pigeonpea [Cajanus cajan (L.) Millsp] ", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.7, Issue 6, page no. pp1521-1539, June 2020, Available at : http://www.jetir.org/papers/JETIR2006554.pdf

Publication Details

Published Paper ID: JETIR2006554
Registration ID: 234763
Published In: Volume 7 | Issue 6 | Year June-2020
DOI (Digital Object Identifier): http://doi.one/10.1729/Journal.24003
Page No: 1521-1539
Country: Ahmednagar, maharashtra, India .
Area: Biological Science
ISSN Number: 2349-5162
Publisher: IJ Publication


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