• Daspute Abhijit Arun

Pigeonpea sterility mosaic disease (PSMD) caused by pigeonpea sterility mosaic virus (PPSMV) is one of the major biotic concerns that leads to serious yield losses and, hence, poses a big challenge for pigeonpea production in the Indian subcontinent. In this study the inheritance, identification and development of molecular markers associated with Bidar isolate of PPSMV resistance gene by using F2 population was attempted. We reveal that PSMD is controlled by two genes, named as SV1 (inhibitory) and SV2 (resistant). Of the 300 RAPD primers, 32 primers recorded polymorphism in the parents, were screened using bulked segregant analysis method. Two markers (IABTPPN18827 and IABTPPAK191295) amplified susceptible parent specific amplicon and were associated with the PSMD responsive gene at a distance of about 12.5 cM and 10 cM respectively. We developed SCAR marker associated with the SV1 gene. Based on the sequencing data of the IABTPPN18827 marker, we successfully developed a dominant SCAR marker, FlntN18827, which was associated with SV1 gene at the distance of 12.5 cM. This SV1-SCAR marker will be a valuable tool for marker-assisted breeding in developing PSMD resistant pigeonpea.

PIGEONPEA, PSMD, PPSMV, SV1, RAPD, SCAR

"Development of a dominant SCAR marker associated with an allele of SV1 gene for sterility mosaic disease resistance in pigeonpea [Cajanus cajan (L.) Millsp] ", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.7, Issue 6, page no.1521-1539, June 2020, Available :http://www.jetir.org/papers/JETIR2006554.pdf

"Development of a dominant SCAR marker associated with an allele of SV1 gene for sterility mosaic disease resistance in pigeonpea [Cajanus cajan (L.) Millsp] ", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.7, Issue 6, page no. pp1521-1539, June 2020, Available at : http://www.jetir.org/papers/JETIR2006554.pdf