• Akshada sharad borhade
• DR. DHOBALE G .S
• DR. JADHAV S.L
• DR DHOBALE S.M

A simple, sensitive and accurate gradient reverse phase high performance liquid chromatography method was developed for determination of dimethyl fumarate in formulation . RP-HPLC method has been developed and validated to live dimethyl fumarate at wavelength ( 210nm)in order to assay. The retention time of dimethyl fumarate was found to be 3.3±0.02 min. the worth of coefficient of correlation between peak area and concentration was found to be 0.9987. The mean percent recovery of dimethyl fumarate in capsules was found within the range of 99.65 to 101.64%. The results of forced degradation studies indicated that the drug was found to be stable in basic, oxidative and thermal conditions while degraded in acidic conditions.The mixture of buffer and ACN ,in the ratio (70:30) used as a mobile phase .It are often conducted from results that the developed HPLC method is straightforward , accurate, precise and specific. Results of stress testing study revealed that the tactic is stability indicating. Thus, this method are often used for routine analysis of dimethyl fumarate capsules and check their stability.

RP-HPLC, Method validation, Stability indicating assay method, Dimethyl fumarate, Simple and accurate method.

"Rp-Hplc Assay Method For Estimation Of Dimethyl Fumarate In Bulk And Capsules", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.7, Issue 6, page no.1695-1704, June 2020, Available :http://www.jetir.org/papers/JETIR2006573.pdf

"Rp-Hplc Assay Method For Estimation Of Dimethyl Fumarate In Bulk And Capsules", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.7, Issue 6, page no. pp1695-1704, June 2020, Available at : http://www.jetir.org/papers/JETIR2006573.pdf